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Plasmid#224364PurposeExpress PheRS protein for his-tagged purificationDepositorInsertPheRS (pheS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-AlaRS
Plasmid#224362PurposeExpress AlaRS protein for his-tagged purificationDepositorInsertAlaRS (alaS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCDC59-Halo
Plasmid#175327PurposeProduction of HaloTag fusion proteinDepositorInsertCCDC59 (CCDC59 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mNeon-Blast
Plasmid#207709PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mNeon-Puro
Plasmid#207720PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mNeonGreen
Plasmid#137802PurposeVisualization of microtubulesDepositorArticleInsertEnsconsin microtubule binding domain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-3xFLAG-Blast
Plasmid#207717PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-mScarlet-Puro
Plasmid#207722PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
p005-RFP-strong
Plasmid#108317PurposeSynthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.DepositorInsertRFP under controlf of strong synthetic constitutive promoter pFAB4005
UseTagsExpressionBacterialMutationPromoterpFAB4005Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mScarlet
Plasmid#207689PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTfR-mNeonGreen
Plasmid#129608PurposeIn vivo visualization of Transferrin receptors (can be used for colocalization studies)DepositorArticleInsertTransferrin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EgI-BlastR
Plasmid#207176PurposeGateway compatible Lentiviral Expression Plasmid EF1a-IRES-BlastRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro
Plasmid#207721PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)-OTI-TCRA_p2_TCRB
Plasmid#131033PurposeExpresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptideDepositorArticleUseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Zeo-dTAG
Plasmid#207705PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti UBC hNLRP3-mNG
Plasmid#218642PurposeExpresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selectionDepositorInsertNLRP3 (NLRP3 Human)
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FAM98A-Halo
Plasmid#175329PurposeProduction of HaloTag fusion proteinDepositorInsertFAM98A (FAM98A Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP2a-Halo
Plasmid#175309PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP2 (IGF2BP2 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL0_20 [panARS-opt ORI]
Plasmid#198933PurposeLevel 0 partDepositorInsertpanARS-opt ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EgI-ZeoR
Plasmid#207178PurposeGateway compatible Lentiviral Expression Plasmid EF1a-IRES-ZeoRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEf1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only