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Plasmid#209005PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH5
Plasmid#199771PurposeExpresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)DepositorInsertcMSPdH5
UseTagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-5'TwStrep
Plasmid#208620PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-B'-l2-B'
Plasmid#209119PurposeBacterial expression of CC-GEMS ligand SUMO-B'-l2-B'DepositorInsertSUMO-B'-l2-B'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'
Plasmid#209115PurposeBacterial expression of CC-GEMS ligand SUMO-A'DepositorInsertSUMO-A'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l3-A'
Plasmid#209118PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l3-A'DepositorInsertSUMO-A'-l3-A'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l2-G'
Plasmid#209122PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l2-G'DepositorInsertSUMO-A'-l2-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l2-B'
Plasmid#209121PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l2-B'DepositorInsertSUMO-A'-l2-B'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l1-A'
Plasmid#209116PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l1-A'DepositorInsertSUMO-A'-l1-A'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-G'-l2-G'
Plasmid#209120PurposeBacterial expression of CC-GEMS ligand SUMO-G'-l2-G'DepositorInsertSUMO-G'-l2-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l4-G
Plasmid#209123PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-GDepositorInsertSUMO-A'-l4-G
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l2-A'
Plasmid#209117PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l2-A'DepositorInsertSUMO-A'-l2-A'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PuRRRE
Plasmid#208323PurposeBacterial expression of the His-tagged peptide PuRRRE.DepositorInsertPuRRRE
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(96-421)
Plasmid#209006PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 96-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80-142)
Plasmid#209012PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28b_His_RAD23A-UBA1
Plasmid#201549PurposeExpresses UBA1 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTags6x HisExpressionBacterialMutationExpresses amino acids 160-205 of DNA polymerase i…PromoterT7Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHM1_ARATH
Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_HALMA
Plasmid#204241PurposeBacterial expression of H. marismortui (Dead Sea halophilic red archaeon) cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_HALMA (mdh Haloarcula marismortui)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHNP_ARATH
Plasmid#204238PurposeBacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHNP_ARATH (AT5G58330 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes not include the chloroplastic transit sequen…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_CHLP8
Plasmid#204242PurposeBacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_CHLP8
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only