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Addgene
Showing: 1241 - 1260 of 16046 results

  1. Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

    Plasmid
    #216125
    Purpose
    Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Insert
    sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216125-plasmid-map-sequence-id-428733

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  2. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid
    #216292
    Purpose
    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-GSG-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    216292-plasmid-map-sequence-id-433349

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  7. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid
    #214230
    Purpose
    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Insert
    cSrc kinase SH2 domain (SRC Human)
    Use
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    214230-plasmid-map-sequence-id-429073

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  8. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Expression
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  9. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP

    Plasmid
    #212107
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27
    Insert
    GABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Entacmaea quadricolor, Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of upst…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    212107-plasmid-map-sequence-id-420139

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  11. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)

    Plasmid
    #218425
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27
    Insert
    GABARAP(G116A)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of down…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    218425-plasmid-map-sequence-id-434958

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  12. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

    Plasmid
    #218426
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27
    Insert
    GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of both…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    218426-plasmid-map-sequence-id-434963

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  16. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

    Plasmid
    #117168
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    117168-plasmid-map-sequence-id-238529

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  18. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

    Plasmid
    #217970
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    217970-plasmid-map-sequence-id-436105

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  19. pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

    Plasmid
    #141452
    Purpose
    B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Cdk6
    Use
    Mouse Targeting; Dre / rox
    Tags
    C-terminal fusion of T2A-TagRFPT-dre-PBD*
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    141452-plasmid-map-sequence-id-315092

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Showing: 1241 - 1260 of 16046 results