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Plasmid#174034PurposeIn vitro transcription of anti-CRISPR AcrIIA4 mRNADepositorInsertAcrIIA4
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-AcrIIA2
Plasmid#174033PurposeIn vitro transcription of anti-CRISPR AcrIIA2 mRNADepositorInsertAcrIIA2
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd SUP
Plasmid#115489PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 CLV3
Plasmid#115485PurposeCRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0+K848A
Plasmid#108296PurposepX459 V2.0 (Plasmid #62988) with the K848A mutationDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEG302 5aa SunTag VP64 gRNA4 (FWA)
Plasmid#115481PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNADepositorInsertg4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 AP3
Plasmid#115484PurposeCRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302 5aa SunTag VP64 gRNA17 (FWA)
Plasmid#115482PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNADepositorInsertg17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv1
Plasmid#221138PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.DepositorInsert3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
UseCRISPRTags3xFLAGExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv2
Plasmid#221139PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).DepositorInsert3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
UseCRISPRTags3xFLAGExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)
Plasmid#179007PurposeSp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)
Plasmid#179006PurposeSp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)
Plasmid#179003PurposeSp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)
Plasmid#179002PurposeSp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)
Plasmid#179001PurposeSp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)
Plasmid#179000PurposeSp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)
Plasmid#178999PurposeSp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-AcrIIC3
Plasmid#174036PurposeIn vitro transcription of anti-CRISPR AcrIIC3 mRNADepositorInsertAcrIIC3
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-AcrIIC1
Plasmid#174035PurposeIn vitro transcription of anti-CRISPR AcrIIC1 mRNADepositorInsertAcrIIC1
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p415:iCas9
Plasmid#138266PurposeExpression of mTN3-GGS6-dCas9 (iCas9) in yeast.DepositorInsertmTN3-GGS6-dCas9
UseCRISPRTagsExpressionYeastMutationCas9-D10A, H840APromoterGalLAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only