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Showing: 1221 - 1240 of 5112 results

  1. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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  7. pFETCh_ZNF3

    Plasmid
    #72362
    Purpose
    Donor vector for 3' FLAG tag of human ZNF3
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    ZNF3 Homology Arms (ZNF3 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    72362-plasmid-map-sequence-id-132112

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  8. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

    Plasmid
    #191390
    Purpose
    Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(1-100)Cy-
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191390-plasmid-map-sequence-id-378098

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  9. pPV476

    Plasmid
    #42929
    Depositor
    James Lok
    Insert
    attR2::egfp (870 bp)::taa::Ss-era-1 terminator (598 bp)::attL3
    Use
    Multi-site gateway donor plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    42929-plasmid-map-sequence-id-58192

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  10. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid
    #191529
    Purpose
    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    H2BK_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191529-plasmid-map-sequence-id-378106

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  11. Rhesus AAVS1-CAG-copGFP

    Plasmid
    #84209
    Purpose
    Rhesus AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP
    Depositor
    Cynthia Dunbar
    Inserts
    Rhesus AAVS1 5’ homology arm
    Rhesus AAVS1 3’ homology arm
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    84209-plasmid-map-sequence-id-166261

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  12. pFHMSP-LIC-C

    Plasmid
    #26100
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    8x His tag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pFHMSPN-avi-TEV-LIC V

    Plasmid
    #220885
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expresssion
    Tags
    Honeybee melittin signal followed by His, avi and…
    Expression
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220885-plasmid-map-sequence-id-436698

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  14. pDONR221-deltaUBA-short-UBASH3A-Ctag

    Plasmid
    #195397
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195397-plasmid-map-sequence-id-388659

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  15. pDONR221-deltaUBA-short-UBASH3A-Ntag

    Plasmid
    #195398
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for N-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195398-plasmid-map-sequence-id-386315

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  17. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

    Plasmid
    #195503
    Purpose
    SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
    Use
    CRISPR; Donor vector
    Tags
    T2A-H2B-mCitrine
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195503-plasmid-map-sequence-id-384989

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Showing: 1221 - 1240 of 5112 results