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Showing: 1221 - 1240 of 14674 results

  10. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  12. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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  16. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Article
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Use
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pAcGP67A-TfR

    Plasmid
    #12130
    Depositor
    Pamela Bjorkman
    Article
    Lebron et al Cell. 1998 Apr 3. 93(1):111-23.
    Insert
    transferrin receptor (TFRC Human)
    Use
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    The portion of the human TfR gene encoding residu…
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  18. pNUT N6His K206E/K534A hTFNG

    Plasmid
    #70133
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.
    Insert
    N6His K206e/K534A hTFNG (TF Human)
    Use
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid and Lys534 to a…
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70133-plasmid-map-sequence-id-129418

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  20. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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Showing: 1221 - 1240 of 14674 results