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Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG
Plasmid#130691PurposeExpresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
UseTags3xFLAG and 3xHAExpressionMammalianMutationE653_G1960delPromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG
Plasmid#130689PurposeExpresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-46.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsM, M1961_E1992delPromoterCMVAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG
Plasmid#130686PurposeExpresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_G1960delPromoterCMVAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG
Plasmid#130690PurposeExpresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsM, G1720_Y1846delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG
Plasmid#130685PurposeExpresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.41-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_Q1684delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG
Plasmid#130682PurposeExpresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationE653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAG
Plasmid#130687PurposeExpresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_E1992delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH50
Plasmid#128212Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK007
Plasmid#128220Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH72
Plasmid#128221Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH73
Plasmid#128222Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH74
Plasmid#128223Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH90
Plasmid#128203PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH104
Plasmid#128209PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC111
Plasmid#118906Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6DepositorInsertNcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
UseTagsLPETGg, HIS6ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω2
Plasmid#101705PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω1
Plasmid#101706PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α2
Plasmid#101707PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α1
Plasmid#101708PurposeGoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω2
Plasmid#101709PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only