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pExchange_tdk_BT1311p-tetR-1

Plasmid

#90325

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGabrg2

Plasmid

#124857

Purpose

Mutagenesis of Gabrg2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg2 (Gabrg2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF1771

Plasmid

#143542

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TBP (TBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM4-Flag

Plasmid

#180409

Purpose

lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM4 (TGM4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM6-Flag

Plasmid

#180411

Purpose

lentiviral transduction of human TGM6 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM6 (TGM6 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM5-V5

Plasmid

#180414

Purpose

lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM5 (TGM5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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ESCRT-I

Plasmid

#17634

Depositor

James Hurley

Article

Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

Insert

Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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Plxna2-Fc-His

Plasmid

#72123

Purpose

Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna2 (Plxna2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pNCS mStrawberry

Plasmid

#91768

Purpose

Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Strawberry

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened WT

Plasmid

#126408

Purpose

Full length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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