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Plasmid#131777PurposeRhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR taggingDepositorInsertmsfYFP - 24x MS2 loops
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV Btg2 YFP MS2
Plasmid#131776PurposeBtg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR taggingDepositorInsertmsfYFP - 24x MS2 loops
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDM304_YFP-IqgC_mRFP-Raf1(RBD)
Plasmid#128698Purposesimultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cellsDepositorInsertsiqgC (full length)
Raf1 kinase (aa 50-132)
UseDictyostelium discoideum expression vectorTagsYFP and mRFPExpressionMutationnonePromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC601: pMVP (L3-L2) eYFP + polyA
Plasmid#121769PurposepMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.DepositorInserteYFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD001: pMVP (L3-L2) eYFP + WPRE
Plasmid#121777PurposepMVP L3-L2 entry plasmid, contains eYFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest in lentivirus vectors.DepositorInserteYFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO401: pMVP (R4-R3) eYFP open
Plasmid#121737PurposepMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteYFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_R89A-PolyA
Plasmid#112291PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Arginine 89 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_L91A-PolyA
Plasmid#112292PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Leucine 91 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_F95A-PolyA
Plasmid#112293PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Phenylalanine 95 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW sYFP2-T2A-mTurquoise2
Plasmid#118436PurposeProduces equimolar expression of sYFP2 and mTq2DepositorInsertsYFP2-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mYFP
Plasmid#105138PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mYFP
UseTags3nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hGCN5-NLS
Plasmid#103827PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorUseTagsExpressionMammalianMutationhGCN5: A566G (E189G), G585T (K195N), G726A (silen…PromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL4-YFPc-1
Plasmid#102396Purposesplit YFP. Plant expression of CBL4-YFPc-1DepositorInsertCBL4 (SOS3 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL10-YFPc-1
Plasmid#102402Purposesplit YFP. Plant expression of CBL10-YFPc-1DepositorInsertCBL10 (CBL10 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL9-YFPc-1
Plasmid#102400Purposesplit YFP. Plant expression of CBL9-YFPc-1DepositorInsertCBL9 (CBL9 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL8-YFPc-1
Plasmid#102399Purposesplit YFP. Plant expression of CBL8-YFPc-1DepositorInsertCBL8 (CBL8 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL6-YFPc-1
Plasmid#102398Purposesplit YFP. Plant expression of CBL6-YFPc-1DepositorInsertCBL6 (CBL6 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL5-YFPc-1
Plasmid#102397Purposesplit YFP. Plant expression of CBL5-YFPc-1DepositorInsertCBL5 (CBL5 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL1-YFPc-1
Plasmid#102393Purposesplit YFP. Plant expression of CBL1-YFPc-1DepositorInsertCBL1 (CBL1 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CIPK5-YFPn-1
Plasmid#102392Purposesplit YFP. Plant expression of CIPK5-YFPn-1DepositorInsertCIPK5 (CIPK5 Mustard Weed)
UseTagsYFPnExpressionPlantMutationPromoter35SAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only