Skip to main content
Addgene
Showing: 1201 - 1220 of 4474 results

  1. pYL237

    Plasmid
    #173194
    Purpose
    CRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene
    Depositor
    Thomas Cech
    Article
    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:
    Insert
    EZH2 RNA-binding mutant
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    173194-plasmid-map-sequence-id-360541

    Enlarge

  2. KG#471

    Plasmid
    #110935
    Purpose
    Expresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tail
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2015 Sep;201(1):91-116. doi: 1
    Inserts
    mig-13 promoter
    unc-54 3' control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110935-plasmid-map-sequence-id-212600

    Enlarge

  3. KG#244

    Plasmid
    #110927
    Purpose
    Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord
    Depositor
    Kenneth Miller
    Article
    Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.
    Inserts
    unc-129 promoter
    unc-54 3' control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110927-plasmid-map-sequence-id-212562

    Enlarge

  4. KG#80

    Plasmid
    #110875
    Purpose
    Expresses the C. elegans acy-1 cDNA in body wall muscle
    Depositor
    Kenneth Miller
    Article
    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1
    Inserts
    myo-3 promoter
    acy-1 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110875-plasmid-map-sequence-id-212559

    Enlarge

  6. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #42230
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42230-plasmid-map-sequence-id-419908

    Enlarge

  7. pAcGFP1-mito

    Plasmid
    #174180
    Purpose
    Express AcGFP1 with mitochondria-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Article
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Insert
    AcGFP with mitochondria-targeting sequence
    Use
    Tags
    the mitochondria-targeting sequence of the cytoch…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    174180-plasmid-map-sequence-id-354789

    Enlarge

  9. KG#121

    Plasmid
    #110879
    Purpose
    Expresses the C. elegans unc-31 cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1
    Inserts
    rab-3 promoter
    unc-31 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Missing first 408bp
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110879-plasmid-map-sequence-id-212481

    Enlarge

  13. KG#371

    Plasmid
    #110882
    Purpose
    Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1
    Inserts
    unc-129 promoter
    CTNS-1a-mCherry cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Use
    Tags
    intron-mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110882-plasmid-map-sequence-id-212577

    Enlarge

  18. KG#901

    Plasmid
    #110936
    Purpose
    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110936-plasmid-map-sequence-id-216868

    Enlarge

  19. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42335-plasmid-map-sequence-id-275689

    Enlarge

Showing: 1201 - 1220 of 4474 results