We narrowed to 15,977 results for: LEA

1. pOCC28

   Plasmid

   #118873

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-HiFi-nCas9n-A72

   Plasmid

   #125646

   Purpose

   micro-injection of poly-adenylated HiFi-nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   HiFi-nCas9n
   

   Use

   Rna in vitro transcription vector

   Mutation

   R691A from wild-type Cas9

   Promoter

   T3

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

   Plasmid

   #139788

   Purpose

   Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

   Depositor

   Arneet Saltzman

   Article

   Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

   Type

   Empty backbone

   Tags

   6xHis (cleavable by TEV protease) and GST (cleava…

   Expression

   Bacterial

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-mCherry-TSC2-NLS

   Plasmid

   #192441

   Purpose

   Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2-NLS (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-HA-dSpCas9

   Plasmid

   #92112

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9
   

   Use

   CRISPR

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJOE5751.1

   Plasmid

   #135083

   Purpose

   pBR322-based L-rhamnose-inducible vector.

   Depositor

   Josef Altenbuchner

   Article

   Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

   Insert

   P-rhaP-BAD-His tag-eGFP
   

   Expression

   Bacterial

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB21_TaNAM-A1

   Plasmid

   #124572

   Purpose

   Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TaNAM-A1
   

   Tags

   10xMyc

   Expression

   Plant

   Promoter

   35s

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC193

   Plasmid

   #118903

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease; mGFP and TwinStr…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC121

   Plasmid

   #118893

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC204

    Plasmid

    #118894

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and mKate2, HIS6,…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJOE6089.4

    Plasmid

    #135081

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-eGFP-Strep-tag II
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TALEN-CCR5L18-CtermCan

    Plasmid

    #51438

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) Can C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. TALEN-CCR5R18-CtermCan

    Plasmid

    #51439

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) Can C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC230

    Plasmid

    #118871

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mCitrine, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOCC69

    Plasmid

    #118874

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mGFP, cleava…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX4T-1_OCT4_S235A

    Plasmid

    #40639

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Tags

    GST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…

    Expression

    Bacterial

    Mutation

    S235A

    Promoter

    tac

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

18. TALEN-CCR5L18-NtermN2

    Plasmid

    #51446

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q and K153Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. TALEN-CCR5R18-NtermN2

    Plasmid

    #51447

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N2 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q and K153Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+-Zeo-miR-430-G-G mm

    Plasmid

    #221728

    Purpose

    Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430-G-G mm
    

    Use

    Fish expression

    Mutation

    G-G mismatch of miR-430 site

    Promoter

    simian CMV IE94

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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