We narrowed to 16,030 results for: LEA

1. pREP3-TEV-NLS

   Plasmid

   #21146

   Depositor

   Stephen Kearsey

   Article

   Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

   Insert

   TEV protease (TEVgp1 tobacco etch virus)
   

   Use

   S. pombe

   Tags

   2xNLS, NLS, and myc

   Expression

   Yeast

   Available Since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC305

   Plasmid

   #118898

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and TwinStrep-MB…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTXB1-EXO1b

   Plasmid

   #68267

   Purpose

   Bacterial expression of human EXO1

   Depositor

   Stefano Ferrari

   Article

   El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

   Insert

   EXO1 (EXO1 Human)
   

   Tags

   Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   b form, codons 1-846. Please see depositor commen…

   Promoter

   T7

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pFB-LIC-Bse

   Plasmid

   #26108

   Purpose

   SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   His6 - TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS pEGFP-N3 (1299)

   Plasmid

   #62043

   Purpose

   mammalian expression of NLS-EGFP

   Depositor

   Eric Schirmer

   Article

   Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

   Insert

   SV40 NLS
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pALS099 (pGEX-2TP-cTevHis-DEST)

   Plasmid

   #139787

   Purpose

   Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

   Depositor

   Jeannie Lee, Arneet Saltzman

   Article

   Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

   Type

   Empty backbone

   Tags

   6xHis (cleavable by TEV protease) and GST (cleava…

   Expression

   Bacterial

   Available Since

   April 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-eCas9-sgMIB1

   Plasmid

   #140239

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   sgRNA targeting human MIB1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC72

   Plasmid

   #118866

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease, mGFP, cleavable…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC28

   Plasmid

   #118873

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-HiFi-nCas9n-A72

    Plasmid

    #125646

    Purpose

    micro-injection of poly-adenylated HiFi-nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    HiFi-nCas9n
    

    Use

    Rna in vitro transcription vector

    Mutation

    R691A from wild-type Cas9

    Promoter

    T3

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

    Plasmid

    #139788

    Purpose

    Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Arneet Saltzman

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-mCherry-TSC2-NLS

    Plasmid

    #192441

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2-NLS (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-HA-dSpCas9

    Plasmid

    #92112

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9
    

    Use

    CRISPR

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB21_TaNAM-A1

    Plasmid

    #124572

    Purpose

    Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Tags

    10xMyc

    Expression

    Plant

    Promoter

    35s

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC121

    Plasmid

    #118893

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and MBP, cleavab…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC204

    Plasmid

    #118894

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and mKate2, HIS6,…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJOE6089.4

    Plasmid

    #135081

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-eGFP-Strep-tag II
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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