We narrowed to 16,093 results for: LEA

1. pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

   Plasmid

   #130690

   Purpose

   Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   1M_T1522delinsM, G1720_Y1846del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG

   Plasmid

   #130685

   Purpose

   Expresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-9.41-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   I378_Q1684del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG

   Plasmid

   #130682

   Purpose

   Expresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   E653_G1960del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAG

   Plasmid

   #130687

   Purpose

   Expresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-9.47A-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   I378_E1992del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-RTRG

   Plasmid

   #69845

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   1 RTRG repeat
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-(RTRG)2

   Plasmid

   #69846

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   2 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-(RTRG)3

   Plasmid

   #69847

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   3 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-(RTRG)4

   Plasmid

   #69848

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   4 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-eCas9-sgNT

   Plasmid

   #140238

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TALEN-CCR5L18-CtermQ3

    Plasmid

    #51440

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) Q3 C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K788Q, R792Q and R801Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALEN-CCR5R18-CtermQ3

    Plasmid

    #51441

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) Q3 C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K788Q, R792Q and R801Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. TALEN-CCR5L18-NtermN1

    Plasmid

    #51444

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. TALEN-CCR5R18-NtermN1

    Plasmid

    #51445

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREP3-TEV-NLS

    Plasmid

    #21146

    Depositor

    Stephen Kearsey

    Article

    Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Use

    S. pombe

    Tags

    2xNLS, NLS, and myc

    Expression

    Yeast

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTXB1-EXO1b

    Plasmid

    #68267

    Purpose

    Bacterial expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    T7

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pFB-LIC-Bse

    Plasmid

    #26108

    Purpose

    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    His6 - TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. NLS pEGFP-N3 (1299)

    Plasmid

    #62043

    Purpose

    mammalian expression of NLS-EGFP

    Depositor

    Eric Schirmer

    Article

    Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

    Insert

    SV40 NLS
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pALS099 (pGEX-2TP-cTevHis-DEST)

    Plasmid

    #139787

    Purpose

    Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Jeannie Lee, Arneet Saltzman

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Available Since

    April 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-eCas9-sgMIB1

    Plasmid

    #140239

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    sgRNA targeting human MIB1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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