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Showing: 3001 - 3050 of 23271 results

  1. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

    Plasmid
    #133577
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133577-plasmid-map-sequence-id-274079

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  2. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid
    #133576
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133576-plasmid-map-sequence-id-274071

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  3. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

    Plasmid
    #137864
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137864-plasmid-map-sequence-id-274459

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  4. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #133575
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133575-plasmid-map-sequence-id-274068

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  5. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

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  6. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid
    #137859
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137859-plasmid-map-sequence-id-274442

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  7. pAIP4_1TJE

    Plasmid
    #234203
    Purpose
    Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TJE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234203-plasmid-map-sequence-id-464168

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  8. pAIP4_1HKA

    Plasmid
    #234169
    Purpose
    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1HKA (folK Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234169-plasmid-map-sequence-id-464135

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  9. pHEE-R-kan

    Plasmid
    #222827
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, kanamycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222827-plasmid-map-sequence-id-446477

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  10. pHEE-R-kan5A

    Plasmid
    #222830
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, kanamycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222830-plasmid-map-sequence-id-446480

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  11. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA)

    Plasmid
    #137860
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137860-plasmid-map-sequence-id-274446

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  12. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAAA)

    Plasmid
    #137861
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137861-plasmid-map-sequence-id-274449

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  13. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSA)

    Plasmid
    #137862
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137862-plasmid-map-sequence-id-274455

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  14. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAGSA)

    Plasmid
    #137863
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAGSA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137863-plasmid-map-sequence-id-274456

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  15. pCAGGS-mTagBFP2-T2A-iCreERT2 (PVV)

    Plasmid
    #128172
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PVV N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128172-plasmid-map-sequence-id-247964

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  16. pCAGGS-mTagBFP2-T2A-iCreERT2 (PVGSA)

    Plasmid
    #128173
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PVGSA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128173-plasmid-map-sequence-id-254324

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  17. PB CAG-R-bpA-3xSV40pA-R-td-sfGFP

    Plasmid
    #133579
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133579-plasmid-map-sequence-id-274429

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  18. PB CAG-R-bpA-2xSV40pA-R-td-sfGFP

    Plasmid
    #133578
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133578-plasmid-map-sequence-id-274080

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  19. PB CAG-F-bpA-SV40pA-F-td-sfGFP

    Plasmid
    #133571
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133571-plasmid-map-sequence-id-274056

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  20. pLenti4/V5-DEST-P85

    Plasmid
    #40219
    Depositor
    Lynda Chin
    Insert
    P85 alpha (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  21. pHEE-R-kan5A9si

    Plasmid
    #222836
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, kanamycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222836-plasmid-map-sequence-id-446487

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  22. pHEE-R-hyg

    Plasmid
    #222826
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222826-plasmid-map-sequence-id-446476

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  23. PB Neo* PB

    Plasmid
    #138413
    Purpose
    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Piggybac
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138413-plasmid-map-sequence-id-316185

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  24. pAAV-CMV-iRFP713(V256C)-P2A-EGFP

    Plasmid
    #198071
    Purpose
    The AAV vector for iRFP713(V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(V256C)
    Use
    AAV
    Tags
    P2A-EGFP
    Expression
    Mutation
    V256C mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198071-plasmid-map-sequence-id-389701

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  25. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  29. pAIP4_1JL1

    Plasmid
    #234206
    Purpose
    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1JL1 (rnhA Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234206-plasmid-map-sequence-id-464171

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  30. pAIP4_1AKO

    Plasmid
    #234162
    Purpose
    Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1AKO (xthA Escherichia coli K-12)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234162-plasmid-map-sequence-id-464128

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  31. pHEE-R-bar

    Plasmid
    #222828
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222828-plasmid-map-sequence-id-446478

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  32. pHEE-R-hyg5A

    Plasmid
    #222829
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222829-plasmid-map-sequence-id-446479

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  33. pHEE-R-bar5A

    Plasmid
    #222831
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222831-plasmid-map-sequence-id-446481

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  34. pHEE-R-kan9si

    Plasmid
    #222833
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, kanamycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222833-plasmid-map-sequence-id-446484

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  35. pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

    Plasmid
    #198069
    Purpose
    Mammalian expression of iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Use
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198069-plasmid-map-sequence-id-389877

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  36. pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP

    Plasmid
    #198062
    Purpose
    The lentiviral vector for iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198062-plasmid-map-sequence-id-389830

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  37. pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP

    Plasmid
    #197370
    Purpose
    The lentiviral vector for miRFP670nano(R57C/C86S) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    miRFP670nano(R57C/C86S)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    R57C/C86S mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197370-plasmid-map-sequence-id-389688

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  38. pCSIIbleo-6504-P2A-EGFP

    Plasmid
    #197368
    Purpose
    The lentiviral vector for NIR-GECOHI and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    NIR-GECO2(C18S/I426C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    C18S/I426C mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197368-plasmid-map-sequence-id-389693

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  39. F3 Neo* F3

    Plasmid
    #140235
    Purpose
    F3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Flp/ frt
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140235-plasmid-map-sequence-id-316186

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  40. pB6-ROSA26-DTA-Soriano-rev

    Plasmid
    #125746
    Purpose
    B6J ROSA26 targeting vector homology arms, Soriano configuration, DTA, I-CeuI, reverse cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125746-plasmid-map-sequence-id-247203

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  41. pB6-ROSA26-DTA-Soriano-for

    Plasmid
    #125745
    Purpose
    B6J ROSA26 targeting vector homology arms, Soriano configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    125745-plasmid-map-sequence-id-247134

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  42. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

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  43. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

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  44. pJK592

    Plasmid
    #72386
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72386-plasmid-map-sequence-id-133922

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  45. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid
    #85821
    Purpose
    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    Zf Ubi::cMyc oDam_f_GFPoNLS
    Use
    Tol2 based vector
    Tags
    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…
    Expression
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85821-plasmid-map-sequence-id-170479

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  47. pHEE-R-hyg9si

    Plasmid
    #222832
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222832-plasmid-map-sequence-id-446482

    Enlarge

  48. pHEE-R-hyg5A9si

    Plasmid
    #222835
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222835-plasmid-map-sequence-id-446486

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  49. pHEE-R-bar9si

    Plasmid
    #222834
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222834-plasmid-map-sequence-id-446485

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