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Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG2
Plasmid#162603PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACT-Lex
Plasmid#183149PurposeYeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)DepositorInsertGAL4 AD
UseYeat two-hybridTagsHA tagExpressionYeastMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG3
Plasmid#162604PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pG4
Plasmid#162605PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS461
Plasmid#89064Purposeleu1 integration plasmid with adh1pr:Z3EVDepositorInsertZ3EV artificial transcription factor
UseLeu1 integrating vectorTagsExpressionMutationPromoteradh1Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAEF5
Plasmid#136305PurposePlasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin FleissDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yAT1.03R122KR126K
Plasmid#132782PurposeNon-binding version of the FRET biosensor for ATP measurements in budding yeastDepositorInsertyAT1.03R122KR126K ymTq2-tdTomato
UseTagsExpressionYeastMutationR122K, R126KPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0584
Plasmid#133471PurposeIntegration into fission yeast genome at ura4 locus with natMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
UseTagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3HA-TRP1
Plasmid#41599DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTags3xHA and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yAT1.03
Plasmid#132781PurposeFRET biosensor for ATP measurements in budding yeastDepositorInsertyAT1.03 ymTq2-tdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-Kan
Plasmid#44951PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selectionDepositorInsertyoPSmOrange
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-AVT1 chr I
Plasmid#41898PurposeYeast expression of AVT1.DepositorInsertAVT1 (AVT1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB3405
Plasmid#106166PurposeEasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-VPH2 chr I
Plasmid#41897PurposeYeast expression of VPH2.DepositorInsertVPH2 (VPH2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceFeb. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP-T-CaURA3
Plasmid#44877PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP-T with CaUra3 selectionDepositorInsertyoTagRFP-T
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP-T-SpHis5
Plasmid#44842PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP-T with SpHis5 selectionDepositorInsertyoTagRFP-T
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV0585
Plasmid#133472PurposeIntegration into fission yeast genome at ade6 locus with hphMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only