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Plasmid#140016PurposePlasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG, K46E (Please see depositor comments below)PromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_GFP 150TAG
Plasmid#140017PurposePlasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
DT047A
Plasmid#159408PurposeConstitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.T7-LuxR-pr.LuxR-sfGFP-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromotertet and pLux promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT035A
Plasmid#159397PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoterDepositorInsertpr.tac-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromotertac promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC0x_gRNA_Pos5_Ptet
Plasmid#179336PurposegRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJG103
Plasmid#158533PurposewrmScarlet11 x3 tandem repeatsDepositorInsertwrmScarlet11 x3 tandem repeats
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH-tdGFP
Plasmid#74322PurposepH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiaeDepositorInsertpH-tdGFP
UseYeast taggingTagsnoneExpressionYeastMutationN149Y, Q204HPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT12.G
Plasmid#201000PurposepLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD335
Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGPF 150TAG
Plasmid#154765Purposeplasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11.G
Plasmid#200999PurposepLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT13.G
Plasmid#201001PurposepLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0526
Plasmid#133500PurposeExpression of sfGFP targeted to the nucleus with one NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
DT042A
Plasmid#159403PurposeInducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoterDepositorInsertpr.T5-LacO-6xHis-sfGFP
UseTags6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT14.G
Plasmid#201002PurposepLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only