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Plasmid#175130PurposeLentiviral expression of mouse Rnf121-V5DepositorInsertRnf121 (Rnf121 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Nup62-V5
Plasmid#175116PurposeLentiviral expression of mouse Nup62-V5DepositorInsertNup62 (Nup62 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ROSAr
Plasmid#54324PurposeTALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmidDepositorInsertTALEN for the murine ROSA26 locus
UseMouse Targeting and TALENTagsExpressionMammalianMutationPromoterCAGGAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
CROPSeq-PP7-p65-HSF
Plasmid#196719PurposeCROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_BirA*_FLAG_Ct
Plasmid#194071Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-BirA(R118G)-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-axon-jGCaMP8s-WPRE
Plasmid#186043PurposejGCaMP8s expression in axons under the control of the synapsin promoterDepositorInsertaxon-jGCaMP8s
UseAAVTagsGAP43-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-LifeAct-jGCaMP8m-WPRE
Plasmid#186039PurposeAAV-mediated expression of LifeAct-jGCaMP8m under the synapsin promoterDepositorInsertLifeAct-jGCaMP8m
UseAAVTagsLifeAct-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTre-Tight-luciferase-GFP (467)
Plasmid#65491Purposenegative control for expression assaysDepositorInsertEGFP
UseLuciferaseTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY130 (pcDNA3.1-U6-huBsCpf1)
Plasmid#92306PurposeExpression of humanized BsCpf1 and crRNADepositorInserthuBsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD708 DsRed-Express2-ZF21
Plasmid#138795PurposeConstitutive expression of DsRed-Express2-ZF21 under the CMV promoter.DepositorInsertDsRed-Express2-ZF21
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-axon-jGCaMP8m-WPRE
Plasmid#186042PurposejGCaMP8m expression in axons under the control of the synapsin promoterDepositorInsertaxon-jGCaMP8m
UseAAVTagsGAP43-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY128 (pcDNA3.1-U6-huMb2Cpf1)
Plasmid#92304PurposeExpression of humanized Mb2Cpf1 and crRNADepositorInserthuMb2Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-axon-jGCaMP8f-WPRE
Plasmid#186041PurposejGCaMP8f expression in axons under the control of the synapsin promoterDepositorInsertaxon-jGCaMP8f
UseAAVTagsGAP43-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY129 (pcDNA3.1-U6-huMb3Cpf1)
Plasmid#92305PurposeExpression of humanized Mb3Cpf1 and crRNADepositorInserthuMb3Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY127 (pcDNA3.1-U6-huTsCpf1)
Plasmid#92303PurposeExpression of humanized TsCpf1 and crRNADepositorInserthuTsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24::tagBFP2
Plasmid#124321PurposeExpressing Nuclear Localized tagBFP2DepositorInserttagBFP2 (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-NES-jGCaMP8m-WPRE
Plasmid#186036PurposeAAV-mediated cytosolic expression of jGCaMP8m under the synapsin promoterDepositorInsertNES-jGCaMP8m
UseAAVTagsNES-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-NES-jGCaMP8s-WPRE
Plasmid#186037PurposeAAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoterDepositorInsertNES-jGCaMP8s
UseAAVTagsNES-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_016
Plasmid#107143PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only