Showing: 1181 - 1200 of 16046 results
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Plasmid#185684PurposeLow-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutationK121YPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-XPACK-DNAJC5 (L115R)- HaloTag
Plasmid#205712PurposeMammalian expression of XPACK-DNAJC5 (L115R)-HaloTagDepositorInsertDNAJC5 (DNAJC5 Human)
UseTagsHaloTag and XPACK (GCINSKRKD)ExpressionMammalianMutationL115RPromoterAvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003(K31TAG)
Plasmid#210023PurposeExpression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cellsDepositorInsertEGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
UseTagsExpressionMammalianMutationSpyCatcher003 with K31TAG (Amber stop codon) muta…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-gcry-TagBFP
Plasmid#117777PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-cmlc2-TagBFP
Plasmid#117780PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-Puro-dTAG (BRD4)
Plasmid#91793PurposePITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertPuro-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-BSD (BRD4)
Plasmid#91795PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-BSD
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-Puro (BRD4)
Plasmid#91796PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-Puro
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailabilityAcademic Institutions and Nonprofits only -
pCMV-TAPtagN-PrP-SBP-HsAGO2_AC
Plasmid#148440PurposeMammalian Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
UseTagsExpressionMammalianMutationone silent mutation C840Tand sequence differs in …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV GST mTagBFP
Plasmid#206259PurposeENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.DepositorInsertGTS mTagBFP
UseMultimate/gateway entr 4TagsBFGT1 (Golgi localisation peptide)ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2
Plasmid#190181PurposeFluorescent reporter vector to visualize all of cell cycle phases.DepositorInsertsUseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD Omicron XBB.1.5
Plasmid#214724PurposeExpresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertReceptor-Binding Domain (S SARS-CoV-2)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215010PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)
Plasmid#219690PurposeLevel 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215014PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)
Plasmid#115487PurposeCRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2
Plasmid#219548PurposeNLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.DepositorInsertNLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
UseTagsExpressionMammalianMutationThe (SAGG)x4 linker between GAL4(1-65) and the PC…PromoterCMVd1AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2
Plasmid#170273PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
UseTagsExpressionMammalianMutationSee CommentsPromoterCMVd1AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215001PurposeShuttling vector for a mitophagy reporterDepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1181 - 1200 of 16046 results