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Anti-Neuroligin-3 [N110/29R-rat IgG2a] - Chimeric

Plasmid

#231821

Purpose

Mammalian expression plasmid of anti-Neuroligin-3 (Rat) rat IgG2a R-mab. Derived from hybridoma N110/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 V1104L (TTA)

Plasmid

#223274

Purpose

Encodes SARS-CoV-2 spike V1104L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike V1104L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

V1104L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 H1101D (GAC)

Plasmid

#212427

Purpose

Encodes SARS-CoV-2 spike H1101D for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike H1101D (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

H1101D

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres_W

Plasmid

#147897

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-del1097-1110-NF1144AA-shRNAres_W

Plasmid

#147898

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-NF1144AA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-NF1144AA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation and one non silent mutation R23…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W

Plasmid

#147899

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-R1138A-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation and one non silent mutation R23…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186708

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Promoter

PpsbA2*

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186710

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Promoter

Ptrc.x.tetO2

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pRDAX-110 N-Tag GFP[1] L10 FRB mCherry

Plasmid

#129991

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Plasmid

#83841

Purpose

Fluorescent probe for G1/S transition and M/G1 transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E576A-K623A-Q625S-K627A

Plasmid

#238327

Purpose

ScVip1-536-1107-E576A-K623A-Q625S-K627A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E576A-K623A-Q625S-K627A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pUHD 10-3-human CDK11 p110B D543→N mut. cat.-FLAG

Plasmid

#240139

Purpose

Express CDK11 p110B D543N mutant

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

CDK11 p110 (CDK11A Human)

Tags

FLAG

Expression

Mammalian

Mutation

D543N

Promoter

TRE, CMV

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K558A-K605A-K732A-K817A

Plasmid

#238325

Purpose

ScVip1-536-1107-K558A-K605A-K732A-K817A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K558A-K605A-K732A-K817A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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XZ110 CMV-TO-signal-B2AR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233402

Purpose

_-2 adrenergic receptor-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

B2AR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Plasmid

#205761

Purpose

Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1605-del1079-1110-RNFtoAAA-shRNAres_W

Plasmid

#147886

Purpose

Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W

Plasmid

#147887

Purpose

Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5-CASC3 siRes 110-480 188/218-mut

Plasmid

#158544

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Tags

V5

Expression

Mammalian

Mutation

Amino acids 110-480, C-terminal deletion, F188D, …

Promoter

CMV

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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