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Plasmid#55790PurposeGATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistanceDepositorTypeEmpty backboneUseRNAi; GatewayTagsExpressionMammalianMutationPromotermodified H1 RNA geneAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only
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pLIB-TetR-KRAB-IRES-BLAS
Plasmid#55820Purposeretroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistanceDepositorInsertTetR-KRAB
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-THT III
Plasmid#55791PurposeGATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistanceDepositorTypeEmpty backboneUseRNAi; GatewayTagsExpressionMammalianMutationPromotermodified H1 RNA geneAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB303-Tier1-PhCMV-TetR_VPR
Plasmid#169575PurposeTier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).DepositorInserttetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB301-Tier1-PhCMV-rTetR_VPR
Plasmid#169579PurposeTier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).DepositorInsertreverse tetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEnt R3L2 TetO(fl)-3
Plasmid#27107DepositorInsertTetO promoter
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAB300-Tier1-PhCMV-rTetR
Plasmid#169577PurposeTier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).DepositorInsertreverse tetracycline repressor protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2370-Tier1-PhCMV-TetR_F-type
Plasmid#169573PurposeTier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).DepositorInserttetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPtPBR2
Plasmid#80387PurposeEpisome vector for Phaeodactylum tricornutum (pBR322-based)DepositorTypeEmpty backboneUseSynthetic Biology; ; episomal vector for phaeodac…TagsExpressionMutationPromoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEnt L1L3 EF1a-tTA-2
Plasmid#26803DepositorInsertElongation Factor 1 alpha promoter and tTA transgene
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEx-deletion-tet
Plasmid#172214PurposeFor allelic exchange in tetracyclin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseAllelic exchange in prevotella copriTagsExpressionMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEINO3
Plasmid#179487PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugationDepositorInsertTetR/A
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Sth3)
Plasmid#133317Purposeexpresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedTagsExpressionMutationPromoterxyloseAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-PURO
Plasmid#58246PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selectionDepositorInsertPuro(R)
UseLentiviral and RNAi; Gateway destionation vectorTagsTetR-NLS-T2AExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVCERC-5 Pvan-dcas9 (Sth3)
Plasmid#133319Purposeexpresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedTagsExpressionMutationPromotervanillateAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEx-insertion-tet
Plasmid#172211PurposeFor genetic insertion in tetracyclin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseGenetic insertion in prevotella copriTagsExpressionMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416gT-GalL-Cas9
Plasmid#79903PurposepRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expressionDepositorInsertsCas9
guide RNA (gRNA)
Tetracycline Repressor
UseCRISPRTagsExpressionYeastMutationPromoterGPM1 promoter, GalL, and RPR1 promoter with TetO …Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spa)
Plasmid#133318Purposeexpresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pasteurianus)
UseUnspecifiedTagsExpressionMutationPromoterxyloseAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRosa26 R1-ccdB-R2 RexNeo PI-SceI
Plasmid#24418DepositorInsert
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spy)
Plasmid#133316Purposeexpresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pyogenes)
UseCRISPRTagsExpressionMutationPromoterxyloseAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only