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Plasmid#129092PurposeKI EGFP with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertEGFP and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2_SH3
Plasmid#110016PurposeProtein expression and purification of GRAP2_SH3DepositorInsertGRAP2_SH3 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRE-Lepr_FL-IRES-eGFP
Plasmid#196635PurposeDoxycycline inducible expression of full length mouse Lepr with eGFP markerDepositorInsertLepr (Lepr Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-iCT
Plasmid#129094PurposeKI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertiCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
CALCRL-Tango
Plasmid#66234PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCALCRL (CALCRL Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRE-Lepr_Short-IRES-eGFP
Plasmid#196935PurposeDoxycycline inducible expression of short Lepr (no signaling) with eGFP markerDepositorInsertLepr (Lepr Mouse)
UseLentiviralTagsExpressionMammalianMutationshort isoform, lack of signaling casacadePromoterTREAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CALCRb-Tango
Plasmid#66233PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCALCRb (CALCRL Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2-2/2
Plasmid#91368PurposeProtein expression and purification of human SH3 domain construct GRAP2-2/2DepositorInsertGRAP2-2/2 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2-1/2
Plasmid#91467PurposeProtein expression and purification of human SH3 domain construct GRAP2-1/2DepositorInsertGRAP2-1/2 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0058
Plasmid#143007PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAF (MAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0056
Plasmid#142529PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAF (MAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0057
Plasmid#142530PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAF (MAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)
Plasmid#78507PurposeConstruct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labelingDepositorInsertGads SH3 SH2 SH3 domain (GRAP2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HSP90B1_WT
Plasmid#82130PurposeGateway Donor vector containing HSP90B1 , part of the Target Accelerator Plasmid Collection.DepositorInsertHSP90B1 (HSP90B1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HSPA5_WT
Plasmid#82132PurposeGateway Donor vector containing HSPA5 , part of the Target Accelerator Plasmid Collection.DepositorInsertHSPA5 (HSPA5 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-mLepr-COMP5AP-AviTag-9xHis
Plasmid#157628PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertmLepr (Lepr Mouse)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-mLepr-Fc(DAPA)-AviTag-6xHis
Plasmid#157617PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertLepr (Lepr Mouse)
UseTagsFc(DAPA)-Avi-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Anti-Mortalin [N52A/42R-rat IgG2a]
Plasmid#225423PurposeMammalian Expression Plasmid of anti-Mortalin (Mouse) rat IgG2a R-mAb. Derived from hybridoma N52A/42.DepositorInsertAnti-Mortalin (Mus musculus) recombinant (Mouse) monoclonal antibody. (Hspa9 Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV8)
Viral Prep#44362-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV5)
Viral Prep#44361-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only