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Plasmid#220194Purposemini-Tn7 dCas9 expression vector - LacI/pLac promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterLacI/pLacAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_SNF2L-opt_p48
Plasmid#102243PurposeExpression of human codon optimized human SNF2L and pRbAp48 from pFastBac dualDepositorUseTagsExpressionInsectMutationcodon optimized for expressionPromoterPolyhedrin and p10Available sinceNov. 1, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
JDS246
Plasmid#43861PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promotersDepositorInsertmammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-GFP
Plasmid#198222PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertEGFP
UseLentiviralTagsCodon-optimized puromycin resistance gene, follow…ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-coBCL2L1-G94A
Plasmid#188697Purposeexpress codon optimized BCL-XL drug resistant mutantDepositorInsertcoBCL2L1-G94A (BCL2L1 Human)
UseLentiviralTagsExpressionMutationcodon optimized, G94APromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-coBCL2L1-F97C
Plasmid#188696Purposeexpress codon optimized BCL-XL drug resistant mutantDepositorInsertcoBCL2L1-F97C (BCL2L1 Human)
UseLentiviralTagsExpressionMutationcodon optimized, F97CPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9
Plasmid#129391PurposeDerived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.DepositorInsertBurkholderia cenocepacia codon-optimized dCas9
UseCRISPRTagsExpressionBacterialMutationEntire dCas9 codon optimized for the GC-rich B. c…PromoterPrhaBADAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTA231-p.Syn-PAmCherry1Hfx
Plasmid#164661Purposeexpression of H. volcanii codon-optimized PAmCherry1DepositorInsertPAmCherry1Hfx
UseArchaeal expressionTagsExpressionMutationcodon-optimization for Haloferax volcaniiPromoterp.SynAvailable sinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTA231-p.Syn-Dendra2Hfx
Plasmid#164660Purposeexpression of H. volcanii codon-optimized Dendra2DepositorInsertDendra2Hfx
UseArchaeal expressionTagsExpressionMutationcodon-optimization for Haloferax volcaniiPromoterp.SynAvailable sinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG9 (828-839)-ATG101 (1-198)
Plasmid#188073PurposeTo make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cellsDepositorUseTagsGST tagExpressionMammalianMutationcodon-optimized ATG9 (828-839) and codon-optimize…PromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV ArcLightCo (Q239)-T2A-nls-mCherry
Plasmid#85806PurposeGenetically encoded voltage sensor ArcLight- codon optimizedDepositorInsertArcLightCo-Q239
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORFE0005
Plasmid#112073PurposeAtDeadCAS9 D10A H840A without stop codon module CDS1nsDepositorInsertAtDeadCAS9 D10A H840A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-Fluc100-GFPOPT
Plasmid#210596PurposeExpresses SM(FLAG)-optimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-optimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Synthetic_Human_H2AZ.1
Plasmid#42629DepositorInsertHuman H2AZ.1 (H2AZ1 Synthetic, Codon Optimized, Human)
UseTagsUntaggedExpressionBacterialMutationCodon OptimizedPromoterT7Available sinceMarch 21, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6P1 METTL3
Plasmid#200731PurposeBacterial expression of GST-Tagged codon optimized human METTL3DepositorInsertMETTL3 (METTL3 Human)
UseTagsGST from backbone plasmidExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BE4max-P2A-EGFP (pRZ33)
Plasmid#140083PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFPDepositorInsertHuman codon optimized pCAG BE4max with P2A-EGFP
UseTagsP2A-EGFPExpressionMammalianMutationD10A (SpCas9)PromoterCAGAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-HIS-Sangamo
Plasmid#40786Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsN Terminal 6xHISExpressionBacterialMutation+63 C Terminus, Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Sangamo-HIS
Plasmid#40787Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsC Terminal 6xHISExpressionBacterialMutation+63 C Terminus,Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PIV-198}:NosT
Plasmid#212166PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PIV-216}:NosT
Plasmid#212167PurposeThis binary vector expresses mNeonGreen containing the PIV intron (216bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-216}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only