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Plasmid#188695Purposeexpress codon optimized BCL-XLDepositorInsertcoBCL2L1 (BCL2L1 Human)
UseLentiviralTagsExpressionMutationcodon optimizedPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALEN_C63
Plasmid#40788PurposeGolden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsExpressionMammalianMutation+63 C Terminus, Codon Optimized FokI EndonucleasePromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
TALEN_C18
Plasmid#47974PurposeGolden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsExpressionMammalianMutationShortened C-Terminus,Codon Optimized FokI Endonuc…PromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-dCas9
Plasmid#220196Purposemini-Tn7 dCas9 expression vector - XylS/pM promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterXylS/pMAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-dCas9
Plasmid#220194Purposemini-Tn7 dCas9 expression vector - LacI/pLac promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterLacI/pLacAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_OptλHAramp-FLuc100
Plasmid#210577PurposeExpresses Optimal lambda HA-Optimal Firefly luciferaseDepositorInsertOptimal lambda HA-Optimal Firefly luciferase
UseTagslambda HAExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-dCas9
Plasmid#220195Purposemini-Tn7 dCas9 expression vector - AraC/pBAD promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterAraC/pBADAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-dCas9
Plasmid#220193Purposemini-Tn7 dCas9 expression vectorDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
JDS246
Plasmid#43861PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promotersDepositorInsertmammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-GFP
Plasmid#198222PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertEGFP
UseLentiviralTagsCodon-optimized puromycin resistance gene, follow…ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/o35s[]:mNeonGreen-I{PIV-198}:NosT
Plasmid#212165PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with the truncated 35sCAMV promoterDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-coBCL2L1-G94A
Plasmid#188697Purposeexpress codon optimized BCL-XL drug resistant mutantDepositorInsertcoBCL2L1-G94A (BCL2L1 Human)
UseLentiviralTagsExpressionMutationcodon optimized, G94APromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-coBCL2L1-F97C
Plasmid#188696Purposeexpress codon optimized BCL-XL drug resistant mutantDepositorInsertcoBCL2L1-F97C (BCL2L1 Human)
UseLentiviralTagsExpressionMutationcodon optimized, F97CPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9
Plasmid#129391PurposeDerived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.DepositorInsertBurkholderia cenocepacia codon-optimized dCas9
UseCRISPRTagsExpressionBacterialMutationEntire dCas9 codon optimized for the GC-rich B. c…PromoterPrhaBADAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTA231-p.Syn-PAmCherry1Hfx
Plasmid#164661Purposeexpression of H. volcanii codon-optimized PAmCherry1DepositorInsertPAmCherry1Hfx
UseArchaeal expressionTagsExpressionMutationcodon-optimization for Haloferax volcaniiPromoterp.SynAvailable sinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTA231-p.Syn-Dendra2Hfx
Plasmid#164660Purposeexpression of H. volcanii codon-optimized Dendra2DepositorInsertDendra2Hfx
UseArchaeal expressionTagsExpressionMutationcodon-optimization for Haloferax volcaniiPromoterp.SynAvailable sinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas3-APOBEC1
Plasmid#229536PurposepMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsrAPOBEC1
Cas3
Uracil glycosylase inhibitor
UseTagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…PromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG9 (828-839)-ATG101 (1-198)
Plasmid#188073PurposeTo make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cellsDepositorUseTagsGST tagExpressionMammalianMutationcodon-optimized ATG9 (828-839) and codon-optimize…PromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV ArcLightCo (Q239)-T2A-nls-mCherry
Plasmid#85806PurposeGenetically encoded voltage sensor ArcLight- codon optimizedDepositorInsertArcLightCo-Q239
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-Fluc100-GFPOPT
Plasmid#210596PurposeExpresses SM(FLAG)-optimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-optimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only