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Showing: 101 - 120 of 2063 results

  1. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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  2. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  3. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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  4. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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  14. pGL1_110

    Plasmid
    #210694
    Purpose
    Level 1 plasmid, barcoded ORI
    Depositor
    Nili Ostrov, Cultivarium Tools, Charlie Gilbert
    Insert
    pTHT15 with barcode 30
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    210694-plasmid-map-sequence-id-417386

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  16. pGL1_108

    Plasmid
    #210692
    Purpose
    Level 1 plasmid, barcoded ORI
    Depositor
    Nili Ostrov, Cultivarium Tools, Charlie Gilbert
    Insert
    pIM13 with barcode 28
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    210692-plasmid-map-sequence-id-417382

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  17. pGL1_105

    Plasmid
    #210689
    Purpose
    Level 1 plasmid, barcoded ORI
    Depositor
    Nili Ostrov, Cultivarium Tools, Charlie Gilbert
    Insert
    pBP1 ORI with barcode 11
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    210689-plasmid-map-sequence-id-417373

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  18. pGL1_107

    Plasmid
    #210691
    Purpose
    Level 1 plasmid, barcoded ORI
    Depositor
    Nili Ostrov, Cultivarium Tools, Charlie Gilbert
    Insert
    pCD6 ORI with barcode 27
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    210691-plasmid-map-sequence-id-417378

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  19. pGL1_106

    Plasmid
    #210690
    Purpose
    Level 1 plasmid, barcoded ORI
    Depositor
    Nili Ostrov, Cultivarium Tools, Charlie Gilbert
    Insert
    pCB102 ORI with barcode 26
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    210690-plasmid-map-sequence-id-417375

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Showing: 101 - 120 of 2063 results