-
Plasmid#118472PurposecpBFP-based single-color biosensor for monitoring Protein Kinase C activity.DepositorInsertblueCKAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512_Mut235
Plasmid#179278PurposeExpresses Br512_Mut235 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialMutationPromoterT7Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.1
Plasmid#171200PurposeExpresses Br512g3.1 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.2
Plasmid#171201PurposeExpresses Br512g3.2 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
KARS_pNIC-Bio3
Plasmid#153051PurposeExpression construct for producing Avi tagged Lysyl-tRNA synthetaseDepositorInsertKARS (KARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1
Plasmid#61625PurposeFRET-based biosensor for monitoring JNK activityDepositorInsertJNKAR1
UseTagsCitrine and ECFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
EKARet-nuc
Plasmid#96896PurposeFLIM sensor for ERK activation in nucleusDepositorInsertFLIM Sensor for ERK Activity in Nucleus
UseTagsEGFP and sREAChetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PRKAR1B
Plasmid#23376DepositorInsertPRKAR1B (PRKAR1B Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR2-CR
Plasmid#40255DepositorInsertAKAR2
UseTagsHis6 and XpressExpressionMammalianMutationPromoterCMV IEAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR
Plasmid#140051PurposeGenetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.DepositorInsertABKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SynP_DIO_tAKARα- T391A_WPRE
Plasmid#196676PurposeAAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).DepositorInserttAKARα-T391A
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-blueAKAR
Plasmid#118470PurposecpBFP-based single-color biosensor for monitoring Protein Kinase A activity.DepositorInsertblueAKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR(T/A)
Plasmid#140052PurposeNegative control mutant for ABKAR biosensor.DepositorInsertABKAR(T/A)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GR-AKAR3
Plasmid#173015PurposeImproved green-red FRET-based PKA activity reporter.DepositorInsertGR-AKAR3
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-ABKAR
Plasmid#65068PurposeExpression of ABKAR biosensor targeted to the lysozomeDepositorInsertABKAR biosensor
UseTagsLamp1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
UseTagsNLS (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Golgi-ABKAR
Plasmid#61507PurposeExpression of AMPK biosensor targeted to the GolgiDepositorInsertAMPK Biosensor
UseTagsGolgi-targeting signal (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKAR1/Pur
Plasmid#23105PurposeAllows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid. Contains puromycin resistance geneDepositorTypeEmpty backboneUseRNAiTagsFlagExpressionMammalianMutationPromoterTRE for cDNA; U6 for shRNAAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW AKAR3-EV
Plasmid#182533PurposeExpresses the PKA FRET sensor AKAR3-EV in budding yeastDepositorInsertAKAR3-EV
UseTagsExpressionYeastMutationPromoterPMA1Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AKARet-PSD
Plasmid#114732PurposePSD-targeted AKARetDepositorInsertPSD-targeted AKARet
UseTagsEGFP and N-terminus of PSD-95ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only