Addgene

We narrowed to 9,353 results for: Kars

Showing: 1 - 20 of 9353 results

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pAc5.1-PH-mCherry-Oskar 139-606 isoform A (Short Oskar) (HK195)

Plasmid

#206460

Purpose

Oskar 139-606 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Oskar 139-606 (osk Fly)

Use

Tags

PH mcherry

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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CaMKAR

Plasmid

#205315

Purpose

Fluorescent Reporter for CaMKII Activity

Depositor

Elizabeth Luczak, Mark Anderson

Article

Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

Insert

CaMKAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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EKARdual

Plasmid

#99870

Purpose

Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

EKAR

Use

Tags

ShadowG and mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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AKARdual

Plasmid

#99869

Purpose

Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

AKAR4

Use

Tags

LSSmOrange and mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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ampkar

Plasmid

#35097

Purpose

A fluorescent reporter of AMPK activity and cellular energy stress

Depositor

Lewis Cantley

Article

Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

Insert

ampkar

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

na

Promoter

CMV

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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ER-ABKAR

Plasmid

#61508

Purpose

Expression of AMPK biosensor targeted to the endoplasmic reticulum

Depositor

Jin Zhang, Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

ER targeting sequence from CYP450 2C1 (see commen…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
PM-ABKAR

Plasmid

#61506

Purpose

Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

Depositor

Jin Zhang, Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

Lyn kinase sequence (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
CytoCKAR

Plasmid

#14870

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, NES, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pRK5_tAKARβ

Plasmid

#119914

Purpose

Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARβ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2022

Availability

Academic Institutions and Nonprofits only

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CKAR

Plasmid

#14860

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKAR4α

Plasmid

#119919

Purpose

Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKAR4α

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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aCKAR

Plasmid

#124427

Purpose

FRET-based reporter for monitoring aPKC activity in cells

Depositor

Alexandra Newton

Article

Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

Insert

aCKAR

Use

Tags

mCFP and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKAR5

Plasmid

#149461

Purpose

Broad-Host range expression vector, RK2 and pUC origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

AraC-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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BKAR

Plasmid

#14875

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
CKAR-T/A

Plasmid

#14861

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

T/A

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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MitoCKAR

Plasmid

#14872

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, TOM20(1-33), and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
GolgiCKAR

Plasmid

#14871

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
NucCKAR

Plasmid

#14869

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, NLS, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pKAR1

Plasmid

#23104

Purpose

Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid

Depositor

Randy Poon

Article

Ma et al Nucleic Acids Res. 2007 . 35(4):e22.

Type

Empty backbone

Use

RNAi

Tags

Flag

Expression

Mammalian

Mutation

Promoter

TRE for cDNA; U6 for shRNA

Available since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARα

Plasmid

#119913

Purpose

Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge