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Plasmid#141135Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionBacterialMutationD3 deletion, I32F in Zdk2PromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
Plasmid#63729PurposeTo express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).UseTagsT2AExpressionMammalianMutationcodon optimizedPromoterSFFVAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)
Plasmid#48307DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (AAV8)
Viral Prep#60658-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (#60658). In addition to the viral particles, you will also receive purified pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato plasmid DNA. CAG-driven, Flp-dependent expression of the voltage-gated Na+ channel mNaChBac-T2A-tdTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1αTagsNoneAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Antibody#203884-rAbPurposeAnti-DNA-directed RNA polymerase subunit beta' (E. coli) recombinant mouse monoclonal antibodyDepositorRecommended ApplicationsWestern BlotReactivitySource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailable sinceNov. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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psgRNA Library
Pooled Library#115927PurposeGenome-wide knockdown of E. coli genesDepositorExpressionBacterialSpeciesEscherichia coliUseCRISPRAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Antibody#192032-rAbPurposeAnti-GST recombinant mouse monoclonal antibodyDepositorRecommended ApplicationsWestern BlotReactivitySyntheticSource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailable sinceJan. 27, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pTXB1-FIS
Plasmid#51564PurposeExpression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertFIS
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CRP/CAP
Plasmid#51563PurposeExpression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertCRP/CAP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
psgRNA_EcoWG1
Pooled Library#131625PurposeImproved genome-wide knockdown of E. coli genesDepositorExpressionBacterialSpeciesEscherichia coliUseCRISPRAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcPPK
Plasmid#38334DepositorInsertE. coli polyphosphate kinase (ppk Escherichia coli)
UseTags6xHIS, MBP, and TEVExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKM263-EcPPK
Plasmid#38333DepositorInsertE. coli polyphosphate kinase (ppk Escherichia coli)
UseTags6xHIS, GST, and TEVExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDM4-GLY
Plasmid#49808PurposeEncodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.DepositorInsertsphosphoglycerate kinase
glyceraldehyde-3-phosphate dehydrogenase
pyruvate dehydrogenase subunit E1
dihydrolipoamide acetyltransferase
dihydrolipoamide dehydrogenase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
ecALDH
Plasmid#67921PurposeExpresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coliDepositorInsertEscherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-sfGFP
Plasmid#51562PurposeExpression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)DepositorInsertsuperfolder GFP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CII
Plasmid#51566PurposeExpression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcII
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CI
Plasmid#51565PurposeExpression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcI
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLIC-EcTOP
Plasmid#224463PurposeExpression of recombinant Escherichia coli topoisomerase IDepositorInsertEscherichia coli TopA
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK640
Plasmid#72467PurposeEncodes Escherichia coli evgADepositorInsertresponse regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK641
Plasmid#72468PurposeEncodes Escherichia coli evgA (Plac noncoding)DepositorInsertresponse regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only