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We narrowed to 240 results for: CRISPRA

Showing: 101 - 120 of 240 results

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pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA

Plasmid

#158989

Purpose

Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-SID4X

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pPN447

Plasmid

#137871

Purpose

Expression of gRNA targeting POGZ for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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VPR_dCas9

Plasmid

#134601

Purpose

Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

Depositor

Lasse Ebdrup Pedersen

Article

Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

Insert

VPR-dCas9-2A-ZsGreen-DR

Use

Tags

VPR and ZsGreen1-DR

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCD442

Plasmid

#153024

Purpose

dCas9, MCP-SoxS(R93A/S101A)

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dCas9, MCP-SoxS(R93A/S101A)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Available since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLY152

Plasmid

#130950

Purpose

dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
sfgfp

Use

Synthetic Biology

Tags

ASV tag

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

PpspA-2G6 and Ptet

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCD564

Plasmid

#153026

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Available since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pPN461

Plasmid

#137877

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CaTCH BC1

Plasmid

#154195

Purpose

Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

CaTCH BC1

Use

Lentiviral; Catch barcode labeling

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCK005.6

Plasmid

#153025

Purpose

dCas9, MCP-SoxS(R93A/S101A), J106 scRNA

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dCas9, MCP-SoxS(R93A/S101A), J106 scRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Available since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCD565

Plasmid

#153027

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPN454

Plasmid

#137865

Purpose

Expression of gRNA i1 targeting TCF4 for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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FNLCR CRISPRa Cell Surface

Pooled Library

#207471

Purpose

CRISPR-based activation library with guide RNAs targeting all cell surface proteins.

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only
Dual sgRNA CRISPRa Library

Pooled Library

#1000000193

Depositor

Jonathan Weissman

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

Plasmid

#192160

Purpose

Dual AAV vector AAV-dCas9

Depositor

Sidi Chen

Article

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Insert

Dead Cas9

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPN441

Plasmid

#137873

Purpose

PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPN435

Plasmid

#137867

Purpose

Expression of gRNA i3 targeting TCF4 for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPN434

Plasmid

#137866

Purpose

Expression of gRNA i2 targeting TCF4 for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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