Addgene

We narrowed to 1,345 results for: BirA

Showing: 101 - 120 of 1345 results

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rgef-1p::NmBirA

Plasmid

#79971

Purpose

encodes E.coli biotin-ligase under neuronal promotor for C.elegans

Depositor

Baris Tursun

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Use

Tags

NLS::myc

Expression

Worm

Mutation

Promoter

rgef

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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rgef-1p::NmBirA

Plasmid

#79971

Purpose

encodes E.coli biotin-ligase under neuronal promotor for C.elegans

Depositor

Baris Tursun

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Use

Tags

NLS::myc

Expression

Worm

Mutation

Promoter

rgef

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Kusabira Orange-C1 (KO)

Plasmid

#54792

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Use

Tags

Kusabira Orange

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
Kusabira Orange-C1 (KO)

Plasmid

#54792

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Use

Tags

Kusabira Orange

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
mKusabira Orange-N1

Plasmid

#55046

Purpose

Empty backbone for fusing gene of interest to N-term of mKO

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mKO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
mKusabira Orange-N1

Plasmid

#55046

Purpose

Empty backbone for fusing gene of interest to N-term of mKO

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mKO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA5_FRB-pBirA-HA

Plasmid

#83415

Purpose

proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Insert

FRB-pBirA-HA (MTOR Synthetic, Human)

Use

Tags

HA and pBirA

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRB-pBirA-HA

Plasmid

#83415

Purpose

proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Insert

FRB-pBirA-HA (MTOR Synthetic, Human)

Use

Tags

HA and pBirA

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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BirA in pET28a (w400-2)

Plasmid

#26624

Depositor

Eric Campeau

Insert

BirA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pEF1a-BirA-V5-neo

Plasmid

#100548

Purpose

Expresses C-terminal V5-tagged BirA biotin ligase

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Insert

C-terminal V5-tagged BirA

Use

Tags

V5, His

Expression

Mammalian

Mutation

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLX307-CRBN-3F-BirA

Plasmid

#220146

Purpose

Expresses CRBN-BirA for CRBN substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

Cereblon (CRBN Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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OPEN HLA-A*02:01-BirA

Plasmid

#215569

Purpose

E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN HLA-A*02:01 BirA (HLA-A Human)

Use

Tags

BirA

Expression

Bacterial

Mutation

Glycine 120 to Cysteine

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL-3F-BirA

Plasmid

#220147

Purpose

Expresses VHL-BirA for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

codon optimized

Promoter

EF-1a

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Use

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Mutation

Promoter

RBS and T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry_pBirA-HA_FKBP8

Plasmid

#154457

Purpose

expresses mCherry_pBirA-HA_FKBP8/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry_pBirA-HA_FKBP8 (FKBP8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mKusabira Orange-C1 (mKO)

Plasmid

#54834

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Use

Tags

mKusabira Orange

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only