Showing: 11961 - 11980 of 15904 results
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Plasmid#79613PurposeExpresses dCas9-SH3 in yeast cellsDepositorInsertSpCas9
UseTagsSH3 domainExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1AvailabilityAcademic Institutions and Nonprofits only -
WasCFP /pQE-30
Plasmid#79858PurposeCyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.DepositorInsertWasCFP
UseTagsHisTagExpressionBacterialMutationCyan fluorescent protein mCerulean with substitut…PromoterT5 promoter / lac operatorAvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-wtSpCas9-H840A
Plasmid#80453PurposeSpCas9 mammalian expression vectorDepositorInsertSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII
Plasmid#80864PurposePhagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G,P
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PM-CaNARi
Plasmid#64850PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.DepositorInsertLyn-CaNARi
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-GCaMP3
Plasmid#64853PurposeCytosol-targeted version of the Ca2+ indicator GCaMP3.DepositorInsertGCaMP3
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SENP2M497A
Plasmid#61088Purposecleave the SUMO off when use PETSUMO vector for express fusion proteinDepositorInsertSENP2M497A catalytic domain (SENP2 Human)
UseTagsHisExpressionBacterialMutationM497A; Catalytic domain is residues 364-589PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
UseTagsNLS (see comments)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV2-hNCOA6:V5
Plasmid#63585PurposeA subunit of the Trithorax-related (Trr) methyltransferase complex.DepositorInsertNCOA6 (NCOA6 Human)
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pKF251
Plasmid#52532PurposeDouble-GFP reporter for DNA cleavageDepositorInsertDouble-GFP
UseTagsExpressionInsectMutationPromoterubiquitinAvailabilityAcademic Institutions and Nonprofits only -
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pHL1756
Plasmid#53036PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pConNoHindM12AvailabilityAcademic Institutions and Nonprofits only -
pHL1757
Plasmid#53037PurposePconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterPconNoHindM2 and pConNoHindAvailabilityAcademic Institutions and Nonprofits only -
P2-ECFP-pA (Construct 6)
Plasmid#55198PurposeSynthetic promoter responsive to gRNA2DepositorInsertmCerulean
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-NLS-ZapCmR1.1
Plasmid#59011PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR1.1 genetically encoded Zn(II) sensor
UseTagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 11961 - 11980 of 15904 results