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Plasmid#20557DepositorInsertPAPSS1 (PAPSS1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag VRK3
Plasmid#20664DepositorInsertVRK3 (VRK3 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_p53(R280K)_V5
Plasmid#136525PurposeUsed as a donor vector to clone into pSLIKDepositorInsertp53(R280K) (TP53 Human)
UseEntry vector for gateway cloningTagsV5ExpressionMutationp53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Ralstonia-GG-Kit
Plasmid Kit#1000000142PurposeBackbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniquesDepositorAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-GB1
Plasmid#112565PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-GB1 and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLX-PGK-hCDK4
Plasmid#114314PurposeConstitutive expression of the CDK4 proteinDepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cG
Plasmid#100811Purposeexpress rabies virus CVS N2c strain glycoprotein gene (N2cG)DepositorInsertrabies virus CVS N2c strain glycoprotein gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSV40-mCherry-P2A-Hygro
Plasmid#106478PurposeExpression of mCherry and Hygromycin resistance gene from SV40 promoterDepositorInsertsmCherry
Hygromycin B resistance
UseTagsExpressionMammalian and PlantMutationPromoterSV40 promoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-C1
Plasmid#54495PurposeLocalization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517DepositorTypeEmpty backboneUseTagsPA-mCherry1ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 His6-MBP TEV Ura S. cerevisiae expression vector (12URA-C)
Plasmid#48305DepositorTypeEmpty backboneUseNaTagsHis6 and MBPExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-Cre-PGK-Puro
Plasmid#108543PurposeLentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoterDepositorInsertscre recombinase
Puromycin
UseTagsExpressionMutationPromoterEF1apha and PGKAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-N1
Plasmid#54597PurposeLocalization: N1 Cloning Vector, Excitation: 440, Emission: 620DepositorTypeEmpty backboneUseTagsmKeima-RedExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyTagsExpressionMutationBsaI site removedPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-C1
Plasmid#54546PurposeLocalization: C1 Cloning Vector, Excitation: 440, Emission: 620DepositorTypeEmpty backboneUseTagsmKeima-RedExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pExp-Sumo
Plasmid#112574PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
HRE:GFP-pGK:Cre
Plasmid#68552PurposeLentivirus expressing hypoxia-responsive GFP reporter and CreDepositorInsertsGreen Fluorescent Protein
Cre
UseLentiviralTagsExpressionMammalianMutationPromoterHRE and pGKAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR ARRET
Plasmid#111384PurposeARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequencesDepositorInsertARRET
UseGateway entry cloningTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSRKBB-empty
Plasmid#59449PurposeContains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotermodified lacAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pExp-MBP
Plasmid#112568PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-MBP and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcA
Plasmid#107876PurposeExpresses E. coli RtcA in bacterial expression cellsDepositorInsertE. coli RtcA (rtcA Escherichia coli)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only