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Plasmid#100148PurposeExpresses EIF3EHDepositorInsertEIF3H (EIF3H Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pEasyG3_hph
Plasmid#184919PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST NET1A V5 WT
Plasmid#69817PurposeExpresses NET1A-V5 in mammalian cellsDepositorInsertNET1A (NET1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CK2alpha V66A/I174A-HA (pJD23)
Plasmid#37362DepositorInsertCK2α V66A/I174A (CSNK2A1 Human)
UseTagsHA X3ExpressionMammalianMutationV66A, I174APromoterCMVAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
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gRNA-hIRF-1 #12/pSIR
Plasmid#61080PurposeExpresses a guide RNA (gRNA) to target human IRF-1 promoterDepositorInsertgRNA_hIRF1 promoter #12 (IRF1 Human)
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Ubac2
Plasmid#123597DepositorInsertubiquitin associated domain containing 2 (Ubac2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DestFLAGHA_RBPMS
Plasmid#65743Purposeconstitutively expresses RBPMS in mammalian cellsDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1-6xHis-TEV-Nsp8
Plasmid#201022PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti ABE7.10-N-AIDmax
Plasmid#157949PurposeLentiviral expression of ABE7.10-N-AIDmaxDepositorInsertLenti ABE7.10-N-AIDmax
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mH33B-mClover3
Plasmid#171496PurposeT7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNADepositorInsertH3.3B (H3f3b Mouse)
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast
Plasmid#166413Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertProtection Of Telomeres 1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationΔOB deleted 125 a.aPromoteref1-aAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSExpressionMutationPromoterEF1a and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2CE1-P1Egfp
Plasmid#90153Purposecontains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFPDepositorInsertlmo2E1P1Egfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT2res
Plasmid#106301PurposeExpresses SHMT2DepositorInsertserine hydroxymethyltransferase 2 (SHMT2 Human)
UseRetroviralTagsExpressionMammalianMutationSilent mutations destroy sgRNA targeting sitesPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75232PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-d
Plasmid#136295Purposeexpresses ABE8.8-d in mammalian cellsDepositorInsertABE8.8-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpRY dCas9 VPR
Plasmid#188511PurposeExpresses FLAG tagged SpRY dCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only