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Plasmid#157686Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp5 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pORANGE Cplx2-GFP KI
Plasmid#131476PurposeEndogenous tagging of Complexin2: C-terminal (amino acid position: L128)DepositorInsertgRNA and GFP donor (Cplx2 Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-Itprip-V5
Plasmid#120241PurposeExpresses V5-tagged Itprip in lentiviral vectorDepositorInsertItprip (ITPRIP Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (787-1899)
Plasmid#170145PurposeExpresses residues 787-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-786; C-termin…PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (787-2138)
Plasmid#170146PurposeExpresses residues 787-2138 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-786; C-termin…PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Orf6
Plasmid#157727Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoterDepositorInsertFH-Orf6 (ORF6 SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMB72
Plasmid#73088PurposeC-terminal GFP-2xTEV-Avi vector, for fosmid recombineeringDepositorInsertGFP-Avi tag
UseTagsGFP and AviExpressionWormMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE4254
Plasmid#89050PurposeExpresses St chimeric gRNA and human codon-optimized StCas9DepositorInsertsSt chimeric gRNA
hStCas9
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-tTA
Plasmid#127091PurposeAn AAV genome with Cre-dependent expression of tTA from the CAG promoterDepositorInserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterCAGAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FAM86A
Plasmid#85117Purposebacterial expression of FAM86ADepositorInsertEEF2KMT (FAM86A Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ArchT-GFP
Plasmid#63141PurposeAAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.DepositorInsertArchT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Y587E EphA2 pcDNA3
Plasmid#102730PurposeExpression of EphA2 in mammalian cellsDepositorInsertEphA2 (Epha2 Mouse)
UseTagsExpressionMammalianMutationY587E (TAT > GAG)PromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5
Plasmid#112840Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationFull length Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX nsp1 K164A/H165A CoV2
Plasmid#175514PurposeFor protein expression of K164A/H165A nsp1 CoV2DepositorInsertK164A/H165A nsp1 CoV2
UseTagsGST tagExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX nsp1 R124A/K125A CoV2
Plasmid#175513PurposeFor protein expression of R124A/K125A nsp1 CoV2DepositorInsertR124A/K125A nsp1 CoV2
UseTagsGST tagExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX C_K nsp1 K164A/H165A CoV2
Plasmid#175517PurposeExpresses C_K for bioconjugation on nsp1DepositorInsertC_K nsp1 CoV2
UseTagsGST tagExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
NPRL2_pLX307
Plasmid#98364PurposeLentiviral expression of NPRL2DepositorInsertNPRL2 (NPRL2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV N-term HA-FLAG puro BRD7
Plasmid#41934DepositorInsertBRD7 (BRD7 Human)
UseRetroviralTagsHA-FlagExpressionMammalianMutationPromoterAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a-EGFP
Plasmid#205309PurposeProduction of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoterDepositorInsertWHaloCaMP1a-EGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED18
Plasmid#15419DepositorInsertMED18 (MED18 Human)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only