Addgene

We narrowed to 17,066 results for: gateway

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pCellFree_G03 STAT3

Plasmid

#67120

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT3 (STAT3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF1-Myc

Plasmid

#67348

Purpose

Mammalian expression of hArf1 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL2-GFP

Plasmid

#67396

Purpose

Mammalian expression of hArl2 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 HDAC1

Plasmid

#67101

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

HDAC1 (HDAC1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
actc1b-mKate2-rab8b

Plasmid

#109658

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab8b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab8b (rab8b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab34a

Plasmid

#109625

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab34a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab34a (rab34a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab1ab

Plasmid

#109606

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab1ab

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab1ab (rab1ab Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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cARA12-mRFP

Plasmid

#181961

Purpose

ARA12 protein tagged with mRFP, under control of TBA2 promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

ARA12 (ARA12 Mustard Weed)

Use

Tags

mRFP

Expression

Plant

Mutation

Promoter

TBA2

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-actn2-mKate2

Plasmid

#109751

Purpose

Muscle specific zebrafish expression of mKate2 fused to alpha actinin 2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

alpha actinin 2

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2E1

Plasmid

#67089

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2E1 (GTF2E1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
actc1b-BTK-mKate2

Plasmid

#109501

Purpose

Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-BTK-mKate2

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCPP3373

Plasmid

#128718

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopQ1-1

Use

Tags

Expression

Bacterial

Mutation

Synonymous point mutation in the wobble position…

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLIX402 caPHF2

Plasmid

#91701

Purpose

inducible lentiviral expression of constitutively active PHF2

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

PHF2 (PHF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

caPHF2; S757D, S899D, S954D, S1056D

Promoter

TRE promoter, Tet ON

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only
MAC-GPR78

Plasmid

#158382

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GPR78 (GPR78 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCellFree_G03 GATA1

Plasmid

#67068

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-2XHA-Src-KD

Plasmid

#140312

Purpose

Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUWR-Cad-Venus

Plasmid

#58328

Purpose

Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-Venus

Use

Tags

Expression

Insect

Mutation

Promoter

poly-ubiquitin

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pTC-ApoE-Tet-YAP

Plasmid

#85580

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Inserts

CreER
YAP

Use

Cre/Lox

Tags

Flag (2x)

Expression

Mammalian

Mutation

Promoter

ApoE.HCR.hAAT and TRE

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4R3_T CTP

Plasmid

#171736

Purpose

ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal

Use

Multisite gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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PB-Gata2

Plasmid

#60665

Purpose

PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Gata2 (Gata2 Rat)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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