Addgene

We narrowed to 18,528 results for: omp

Showing: 11861 - 11880 of 18528 results

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Expression

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-oScarlet

Plasmid

#137138

Purpose

Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E95D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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BcLOV4 fusion tools screening plasmid #2

Plasmid

#174512

Purpose

Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP

Use

Tags

3xFLAG and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(T27N)-HA

Plasmid

#79426

Purpose

Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 27 to Asparagine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(Q67L)-HA

Plasmid

#79425

Purpose

Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 67 to Leucine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-(BsaI)_CBh-Cas9-T2A-mCherry

Plasmid

#135012

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Use

Tags

3x Flag, 3xFLAG (N terminal on insert), NLS, and …

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

Plasmid

#75118

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

Expression

Mutation

Bromodomain replaced with the bromodomain of BRD7

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
INCTbiosyn-p35S-GFP(rc)13

Plasmid

#127526

Purpose

Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF768

Plasmid

#88524

Purpose

Donor Vector containing ZNF768 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF768 (ZNF768 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-PVR(FL)-FLAG

Plasmid

#157622

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PVR (PVR Human)

Use

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-eGFP-CCR5TC

Plasmid

#135528

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_dCas9_sgRNA NC

Plasmid

#176259

Purpose

pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

dead spCas9

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Expression

Mutation

H840A and D10A mutations on spCas9 to inactivate …

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ROBO4-Fc(DAPA)-AviTag-6xHis

Plasmid

#156864

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ROBO4 (ROBO4 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-sRGECO

Plasmid

#137127

Purpose

Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX CblB-SH2

Plasmid

#46413

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

CblB (CBLB Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 42-359

Promoter

Tac

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-PGK-Puro-IRES-GFP

Plasmid

#75124

Purpose

Retroviral expression plasmid encoding wild type murine Myc

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Tags

None

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
mcry1K31R HA tag

Plasmid

#110297

Purpose

mammalian expression of full length mouse CRY1 mutant K31R

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

mcry1 K31R (Cry1 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

31 Lysines are changed to Argenine

Promoter

CMV

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-358-iRFP670

Plasmid

#110694

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mutation

Truncation of CPSF6 isoform at amino acid 358

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

Plasmid

#32008

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

Promoter

CMV, SP6

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E95D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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