Expressing Nuclear Localized mOrange

Expressing Nuclear Localized tagBFP2

Mammalian expression of Fluorescent CPI-17 phospho mimetic nuclear

Ready-to-use AAV PHP.eB particles produced from AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) (#214858). In addition to the viral particles, you will also receive purified AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) plasmid DNA. minBG-driven expression of SYFP2 plus a separate nuclear FLAG epitope in striatal cholinergic neurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Localization: Nuclear Membrane , Excitation: 487, Emission: 509

p3E entry vector for Three Multisite Gateway

Localization: Nuclear Pore, Excitation: 587, Emission: 610

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag

Express tdTomato when FLP is expressed by another vector

Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag

Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression

Encodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expression

Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)

MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.