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Plasmid#190158PurposeRegulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneUseTagsHis6 tagExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCrepusculo
Plasmid#190156PurposeRegulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneUseTagsHis6 tagExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Arp2/3_pBIG2abcd
Plasmid#173683PurposeInsect expression of the 7-subunit Human Arp2/3 complexDepositorInsertsArp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SPb
Plasmid#83205Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertTomato ACTIN promoter
UseTagsExpressionPlantMutationPromoterTomato ACTINAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti GFAABC1D-dCas9-KRAB-MeCP2
Plasmid#194701PurposeExpresses dCas9-KRAB-MeCP2 in AstrocytesDepositorInsertGFAABC1D promoter
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationNonePromoterGFAABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS8: pTns(AvCAST)
Plasmid#168141PurposeInducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.DepositorInsertsAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT85_PS-mEos3.2_GentaR
Plasmid#173895PurposeTransposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoterDepositorInsertmEos3.2
UseTagsExpressionBacterialMutationPromoterSpiralinAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W
Plasmid#112927PurposeCRISPR-a reporter assay lentiviral vector (gTetO)DepositorInserthU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
OA984
Plasmid#120363PurposeExpresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporterDepositorInsert1C19 single chain antibody
UseUnspecifiedTagsExpressionMutationPromoterAAEL010782 carboxypeptidase A (CPA) gene PromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ubc-megf10-GFP
Plasmid#40207DepositorInsertMegf10 (Megf10 Mouse)
UseTagsGFP fusionExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTK312
Plasmid#59569PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak EGFP ochre attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGAL
Plasmid#60337PurposeContains full length native galactose promoterDepositorInsertspromoter
yECitrine
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1
Plasmid#191675Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30ADepositorUseLentiviralTags6X his and flagExpressionMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-StagRFP-EKAR-EV
Plasmid#173019PurposeRed/far-red FRET-based ERK activity reporter for multiplexed imaging applications.DepositorInsertsmURFP-StagRFP-EKAR-EV
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-β-chain
Plasmid#133731PurposeExpresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.DepositorInsertsb-Chain_gePSI
ZsGreen1
UseTagsExpressionMammalianMutationOnly one part of the gePSI-system (startcodon + a…PromoterpTRE3G-BiAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-mTagBFP-CAAX
Plasmid#75176PurposeMembrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusionsDepositorInsertmTagBFP-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbPylRS(IPYE)
Plasmid#104072PurposeExpression plasmid encoding MbPylRS(IPYE) and pylTDepositorInsertPlpp MbPylRS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b-6h-GST-TEVp
Plasmid#172887PurposeCodon optimized (E. coli) TEVp fused with GST and his-tagged for purificationDepositorInsertTEV protease
UseTagsGST solubility tag and HHHHHHExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACT5FVMF(f)
Plasmid#110549PurposeControl plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008DepositorInsertEGFP, FV LTRs
UseQpcr control for titering foamy virusTagsExpressionMutationPromoterpromoter: MLV LTRAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only