Addgene

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Showing: 11821 - 11840 of 14654 results

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pAAV.GFAP.SV40.eGFP.SV40(polyA)

Plasmid

#195558

Purpose

Expresses eGFP under control of short GFAP promoter

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

eGFP

Use

AAV

Tags

No tags

Expression

Mammalian

Mutation

Promoter

short GFAP

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

Plasmid

#159129

Purpose

expresses NRF2 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

NRF2 (NFE2L2 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-Dual-iCas9-CMV

Plasmid

#167500

Purpose

Expression of dual inducible Cas9 with CMV promoter

Depositor

Jake Liang

Article

Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

Cas9

Use

CRISPR, Lentiviral, and Luciferase

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pH1R-mCherry-N1

Plasmid

#84325

Purpose

Histamine-1 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R (HRH1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD10

Plasmid

#195848

Purpose

Expresses human FZD10 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD10 (FZD10 Human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pR2Q024y

Plasmid

#101022

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Use

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

cmV

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Meis1

Plasmid

#131605

Purpose

expression of mouse Meis1 in mammalian cells

Depositor

Filipe Pereira, Kateri Moore

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Meis1 (Meis1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-Dual-iCas9-hALB

Plasmid

#167502

Purpose

Expression of dual inducible Cas9 with hALB promoter

Depositor

Jake Liang

Article

Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

Cas9

Use

CRISPR, Lentiviral, and Luciferase

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

hALB

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK p2A mCherry

Plasmid

#117691

Purpose

GfaABC1D-iBARK-p2A-mCherry: Viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

AAV

Tags

HA / FLAG

Expression

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-NLS-mRuby2

Plasmid

#99130

Purpose

An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV8, and AAV9

Insert

NLS-mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mDLX5/6

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHS15 (hANGPTL3-Strep)

Plasmid

#109112

Purpose

Expresses StrepII-tagged human ANGPTL3 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL3 (ANGPTL3 Human)

Use

Tags

StrepII

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2

Plasmid

#136235

Purpose

Expresses human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

Insert

human Argonaute-2 (AGO2 Human)

Use

Tags

Strep Sumo

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF1

Plasmid

#214671

Purpose

Bacterial expression of GST-tagged mouse GTSF1

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF1 (Gtsf1 Mouse)

Use

Tags

6x His and GST

Expression

Bacterial

Mutation

Promoter

CspA

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Article

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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xCas9

Plasmid

#138565

Purpose

Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

xCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_Smc4-mAID-HaloTAG

Plasmid

#217658

Purpose

Repair template to endogenously tag SMC4 with mAID-Halo Tag

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

SMC4-mAID-HaloTag (SMC4 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Use

Tags

10xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pH2R-mCherry-N1

Plasmid

#84326

Purpose

Histamine-2 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H2R (HRH2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-MOSPD2 WT

Plasmid

#186467

Purpose

Expression of human MOSPD2 fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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