-
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
UseTagsExpressionBacterialMutationPromoterownAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pGL3 BRE Luciferase
Plasmid#45126DepositorInsertId1-BRE
UseLuciferase; Bmp/smad transcriptional reporterTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-ZnF-hSyn-DIO-PSD95FingR-mGreenLantern
Plasmid#200278PurposeGenetically encoded fluorescent probe for labelling endogenous excitatory synapses in vivoDepositorArticleInsertZnF-FingR-PSD95-mGreenLantern
UseAAVTagsmGreenLanternExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ZnF-hSyn-DIO-GPHNFingR-eGFP
Plasmid#200279PurposeGenetically encoded fluorescent probe for labelling endogenous inhibitory synapses in vivoDepositorArticleInsertzFn-FingR-GPHN-eGFP
UseAAVTagseGFPExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)
Plasmid#24859DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationFull length.PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-AlaRS
Plasmid#224362PurposeExpress AlaRS protein for his-tagged purificationDepositorInsertAlaRS (alaS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECFP-DEVDR-Venus
Plasmid#24537DepositorInsertECFP-DEVDR-Venus
UseTagsECFP and VenusExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
NW11
Plasmid#133443PurposeCovalently circularized nanodisc NW11 for studying membrane proteins and viral entry.DepositorInsertNW11
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOlyA(WT)
Plasmid#132391PurposeOstreolysin A for sensing sphingomyelin/cholesterol complexesDepositorInsertOstreolysin A wild-type
UseTagsHis6 tagExpressionBacterialMutationMutations: C62S, C94S, S151CPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
NW9
Plasmid#133442PurposeCovalently circularized nanodisc NW9 for studying membrane proteins and viral entry.DepositorInsertNW9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-mRFP-T2A-FKBP-5-ptase
Plasmid#40896DepositorInsertPM-FRB-mRFP-T2A-FKBP-5-ptase
UseTagsExpressionMutationPromoterAvailable sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-Cerulean-T2A-FKBP-5-ptase
Plasmid#40897DepositorInsertPM-FRB-Cerulean-T2A-FKBP-5-ptase
UseTagsExpressionMutationPromoterAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-mi3
Plasmid#159995PurposeExpresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccinationDepositorInsertSpyCatcher003-mi3
UseTagsC-TagExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LM670
Plasmid#191552PurposeLM670_MSG-AGGA-promoter-ccdb-TTCC-GShisDepositorInsertccdb
UseTagsHIS-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only