Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

Bacterial expression and purification of human PCNA

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether

For AAV based expression of nuclear jRGECO1a in neurons in Cre-negative neurons with FLEx switch

Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

Expresses human POT1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells