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pZA8

Plasmid

#158486

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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cre_CD

Plasmid

#66030

Purpose

MoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Recombinase

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCW-CCND1-HA-puro

Plasmid

#212670

Purpose

Express tagged cyclin D1

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

HA

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pVRb01_syn20

Plasmid

#49704

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf01_syn20

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag E)

Plasmid

#16609

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag E) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragE (SmaI->XhoI)

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
px330-UFL1 sgRNA1

Plasmid

#134635

Purpose

contains sgRNA targeting human UFL1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFL1 sgRNA1 (UFL1 Human)

Expression

Mammalian

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk3a

Plasmid

#208985

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk3a (kcnk3a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag D)

Plasmid

#16608

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag D) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragD (PvuII->SmaI). The PvuII site is at -484…

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pMIY-mETV3-Flag

Plasmid

#34579

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

FLAG and IRES-YFP

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-4SA(S109)

Plasmid

#33081

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S61A, S127A, S164A, S381A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

Plasmid

#129650

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGoldyTALEN-VIM-TAL-2

Plasmid

#62852

Purpose

TALEN mediated gene editing at human VIM locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Expression

Mammalian

Promoter

miniCAGG

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGoldyTALEN-VIM-TAL-1

Plasmid

#62855

Purpose

TALEN mediated gene editing at human VIM locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Expression

Mammalian

Promoter

miniCAGG

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pSG020

Plasmid

#126503

Purpose

Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHis

Depositor

Ute Romling

Article

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpGgi with 6xHis-tag

Tags

C-terminal 6xHis

Expression

Bacterial

Promoter

PBAD

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Venus wGBD

Plasmid

#85222

Purpose

Venus tagged Cdc-42 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

wGBD (WASL Human)

Tags

Venus

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4107-4303)-GST

Plasmid

#83456

Purpose

Human PC1-CTp30 expression cassette with a GST-tag (678)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97wt-mycStrep

Plasmid

#31837

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pRJPaph-lacZYA

Plasmid

#67037

Purpose

Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
lacZYA

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP5H

Plasmid

#188061

Purpose

PROSS-designed versatile peroxidase 5H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

43 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBS SK mCherryROSAbsr

Plasmid

#54321

Purpose

Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

Depositor

Silvestro Conticello

Article

Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

Insert

mCherry and BlasticidinS-resistance

Use

CRISPR, Mouse Targeting, and TALEN

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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