We narrowed to 26,832 results for: sta

1. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

   Plasmid

   #192386

   Purpose

   To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

   Plasmid

   #192381

   Purpose

   To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

   Plasmid

   #192379

   Purpose

   To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

   Plasmid

   #192377

   Purpose

   To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

   Plasmid

   #192378

   Purpose

   To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDe_Cas9_KANA_R160a

   Plasmid

   #173756

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/R160a_sgRNA2/R160a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6 &StU6

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDe_Cas9_KANA_R160b

   Plasmid

   #173757

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/R160b_sgRNA2/160b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6 &StU6

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDe_Cas9_KANA_R390a

   Plasmid

   #173758

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/390a_sgRNA2/390a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6 &StU6

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Arabidopsis thaliana Atm3 pET21a+

   Plasmid

   #172321

   Purpose

   Expresses AtAtm3 in E.coli

   Depositor

   Douglas Rees

   Article

   Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

   Insert

   Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px459-Daxx KO sgRNA

    Plasmid

    #186939

    Purpose

    Daxx KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Daxx KO sgRNA (Daxx Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC-FLAG.HA.HA.Mm.RAP1.I312R

    Plasmid

    #184643

    Purpose

    Retroviral expressions of mouse RAP1 I312R

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Retroviral

    Tags

    HA FLAG

    Expression

    Mammalian

    Mutation

    I312R

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-CfANLN_sgRNA

    Plasmid

    #183880

    Purpose

    pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    Canis familiaris ANLN sgRNA spacer
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-SU(Z)12(624-900)-H6

    Plasmid

    #183772

    Purpose

    Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

    Insert

    SU(Z)12 residues 624-900 (Su(z)12 Fly)
    

    Use

    Tags

    His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OTUB1/pRetroX-IRES-DsRed#10

    Plasmid

    #180279

    Purpose

    Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    human Otubain-1 (OTUB1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC3-TPK2

    Plasmid

    #176180

    Purpose

    yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2 (TPK2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMC6-spacer

    Plasmid

    #176178

    Purpose

    yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    41 bp non-coding DNA spacer from pYTK048
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMART-Ala2

    Plasmid

    #71326

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    insulated waaHp from E. coli

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+WBP2NL-HA

    Plasmid

    #174791

    Purpose

    Xenopus laevis WBP2NL with 3'HA tag

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    WBP2 N-terminal like (wbp2nl.L Frog)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    aa#165, Y to H; aa# 287, D to H

    Promoter

    SP6

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJet-gR-X4ex2-DTS

    Plasmid

    #177154

    Purpose

    Donor plasmid for knock-in of MT-C34 peptide into exon-2 of CXCR4

    Depositor

    Dmitriy Mazurov

    Article

    Maslennikova et al mBio. 2022 Jan 25:e0358921. doi: 10.1128

    Insert

    MT-C34-pA with CXCR4 arms of homology
    

    Use

    Pcr cloning

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFB_ROSA26_SpCas9_gRNAs

    Plasmid

    #174703

    Purpose

    Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

    Depositor

    Beverly L. Davidson

    Article

    Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

    Insert

    SpCas9 dual gRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge