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Plasmid#111138PurposeFor the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiaeDepositorInsertMSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)
UseTags3x FlagExpressionYeastMutationLeu2 deletionPromoterGal1Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ITGA6-1k-Basic-LUC
Plasmid#107141PurposeLuciferase reporter plasmid to monitor transcriptional activity of canine ITGA6-promoter (1kb fragment)DepositorInsertITGA6-promoter
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD2038
Plasmid#101378PurposeName: pBluescript-ΔRRE. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE.DepositorInsertBlue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE
UseUnspecifiedTagsExpressionMutationBlue Heron Biotechnology Inc. bidirectional mutan…PromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MKRN1 110-482
Plasmid#78747PurposeTo overexpress MKRN1 110-482 in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsHAExpressionMammalianMutation110-482PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MKRN1 1-280
Plasmid#78750PurposeTo overexpress -MKRN1 1-280 in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsHAExpressionMammalianMutation1-280PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-Alpha-0 Dronpa
Plasmid#44193DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsDronpaExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55
Plasmid#59824PurposeAllows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 55Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C49,50,83A
Plasmid#58420PurposeExpresses murine IFITM1-C49,50,83A with an N-terminal HA tag in mammalian cells. (mutated at the 3 evolutionarily conserved cysteines)DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 49, 50, and 83 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49227PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDD2000
Plasmid#101197PurposeName: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.DepositorInsertLANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)
Viral Prep#137141-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Flp-dependent)Available sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP (AAV8)
Viral Prep#137140-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP (#137140). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Flp) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre-dependent)Available sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-iC++-EYFP (AAV8)
Viral Prep#137156-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-iC++-EYFP (#137156). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-iC++-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP (inhibited in presence of Flp) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre-dependent)Available sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP (AAV8)
Viral Prep#137139-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP (#137139). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of ChR2(E123T/T159C)-EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre and Flp-dependent)Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV CD68-hM4D(Gi)-mCherry (AAV1)
Viral Prep#75033-AAV1PurposeReady-to-use AAV1 particles produced from pAAV CD68-hM4D(Gi)-mCherry (#75033). In addition to the viral particles, you will also receive purified pAAV CD68-hM4D(Gi)-mCherry plasmid DNA. CD68-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced microglial inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCD68TagsmCherryAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-PPO-Venus (AAV5)
Viral Prep#139504-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-DIO-PPO-Venus (#139504). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-PPO-Venus plasmid DNA. CAG-driven, Cre-dependent expression of Venus-tagged parapinopsin. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsVenusAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CD68-hM4D(Gi)-mCherry (AAV9)
Viral Prep#75033-AAV9PurposeReady-to-use AAV9 particles produced from pAAV CD68-hM4D(Gi)-mCherry (#75033). In addition to the viral particles, you will also receive purified pAAV CD68-hM4D(Gi)-mCherry plasmid DNA. CD68-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced microglial inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCD68TagsmCherryAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon/Von eYFP (AAV Retrograde)
Viral Prep#137164-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-nEF-Con/Fon/Von eYFP (#137164). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon/Von eYFP plasmid DNA. nEF-driven, Cre, Flp and VCre-dependent expression of EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre, Flp and VCre-dependent)Available sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BM28
Bacterial Strain#201047PurposeMG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.DepositorBacterial ResistanceAmpicillin and kanamycinAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only