-
Plasmid#159400PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoterDepositorInsertpr.J23117-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterJ23117 (weak) promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT038A
Plasmid#159399PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoterDepositorInsertpr.J23106-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterJ23106 (medium) promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT040A
Plasmid#159401PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoterDepositorInsertpr.J23117/J23106-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterhybrid J23117-J23106 promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Akaluc-N1
Plasmid#120371PurposeAkaluc luciferase mammalian expression in -N1 backboneDepositorInsertAkaluc (human optimized)
UseTagsExpressionMammalianMutationHuman codon optimizedPromoterCMVAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorTagsExpressionBacterial and MammalianMutationPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
DT041A
Plasmid#159402PurposeConstitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancerDepositorInsertpLenti-CMV-TrkB (Ntrk2, Mouse) (Ntrk2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-KASH2
Plasmid#187017PurposeInducible disruption of LINC ComplexDepositorInsertKASH2
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluB-NP
Plasmid#165093Purposeprotein expression in E. coliDepositorInsertInfluenza B virus(B/Georgia/05/2019) nucleocapsid protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-KASH2ext
Plasmid#187018PurposeControl for inducible disruption of LINC ComplexDepositorInsertKASH2ext
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
DT044A
Plasmid#159405PurposeInducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)DepositorInsertpr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterT7/LacO promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pSLHDR02_EGFP_tCD8
Plasmid#124061PurposeConstruction of 1st donor for scarless genome editingDepositorInsertsEGFP
tCD8
UseMouse Targeting; Human targetingTagsExpressionMutationPromoterHuman UbC promoter modifiedAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrex-n-eGFP
Plasmid#62544PurposeExpression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genomeDepositorInserteGFP
Use(1)trypanosoma cruzi expression of egfp (2) genom…TagsNoneExpressionMutationNonePromoterRibo-HX1Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrex-b-NLS-hSpCas9
Plasmid#62543PurposeExpression of Cas9 in T. cruziDepositorInsertNLS-hSpCas9
UseCRISPR; Trypanosoma cruzi expressionTags3xFLAG and NLSExpressionMutationPromoterRibo-HX1Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAUC40
Plasmid#71298PurposeSuicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Neo
Plasmid#48336PurposeTrasgenically expresses firefly luciferase in T. cruzi with neomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceJuly 20, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMaCTag-P12
Plasmid#120023PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarletDepositorInsertmScarlet
UsePcr templateTagsmScarletExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:Mla1
Plasmid#132355PurposeExpression of the barley Mla1 NLR from the Zea mays ubiquitin promotorDepositorInsertHvMla1
UseTagsExpressionPlantMutationPromoterpZmUBQAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLHDR03_mCherry_tCD19_unique guide
Plasmid#124062PurposeConstruction of 1st donor for scarless genome editing with unique guideDepositorInsertsmCherry
tCD19
UseMouse Targeting; Human targetingTagsExpressionMutationPromoterHuman UbC modifiedAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only