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Plasmid#123867PurposeEncodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK systemDepositorInsertWPRE-Blast (no terminator)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_018
Plasmid#123733PurposeEncodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-frb-D10A-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_019
Plasmid#123734PurposeEncodes tamoxifen activated split-dCAS9 (N-Terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_015
Plasmid#123678PurposeEncodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4-LoxP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHTSHP-ShhN-Cys
Plasmid#121136PurposeExpresses ShhN with a C-terminal cysteine residues in bacteriaDepositorInsertshh (Shh Mouse)
UseTagsHIS, HRV3C site, and MBPExpressionBacterialMutationAmino acid sequence from 26-191, with an addition…PromotertacAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP
Plasmid#120911PurposeExpresses BFP tagged Ptch1-B (V111FL114FI766F)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHA and TagBFPExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, Y…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP
Plasmid#120908PurposeExpresses Ptch1-B (V111FL114FW115A)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP
Plasmid#120907PurposeExpresses Ptch1-B (I766FV111F)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-Y233C-HA
Plasmid#120904PurposeExpresses Ptch1-B (Y233C) for function assaysDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, Y…PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-L234C-HA
Plasmid#120905PurposeExpresses Ptch1-B (L234C)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, L…PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21b-P35
Plasmid#11808DepositorInsertP35
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pStA212
Plasmid#114171PurposeStart-Stop Assembly Level 212 plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAT220
Plasmid#134888PurposeBacterial expressionDepositorInserteGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Cre Stoplight
Plasmid#45150DepositorInsertCre Stoplight
UseTagsDsRed and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cre Stoplight 2.4
Plasmid#37402DepositorInsertCre Stoplight 2.4
UseTagsZsGreen1 and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAT207
Plasmid#134884PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-TEVp
Plasmid#162599PurposeTEV protease under CMV promoterDepositorInsertTEV protease
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
vAS1
Plasmid#198745Purpose3xHA-3'UTRmg105 -pmg153-NATDepositorTypeEmpty backboneUseEndogenous tagging of mimivirus/megavirus genesTagsHA tagExpressionMutationPromoterpmg741Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only