Addgene

We narrowed to 22,987 results for: GFP

Showing: 1161 - 1180 of 22987 results

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px458_2A_GFP_sgRNA_ELAVL2

Plasmid

#106107

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL2 (ELAVL2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_EIF2AK2

Plasmid

#106108

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting EIF2AK2 (EIF2AK2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_PRKRA

Plasmid

#106109

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting PRKRA (PRKRA Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2GFP

Plasmid

#82416

Purpose

3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Has Service

Cloning Grade DNA

Insert

GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EFS (P2A)

Availability

Academic Institutions and Nonprofits only

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pgRGFP

Plasmid

#82695

Purpose

Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK, U6

Availability

Academic Institutions and Nonprofits only

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pACYC.GFP1Amb

Plasmid

#102792

Purpose

Expresses GFP with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Use

Tags

Expression

Bacterial

Mutation

Y39Stop

Promoter

Availability

Academic Institutions and Nonprofits only

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pCAGGS_3E5E_eGFP

Plasmid

#103054

Purpose

Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

Ebola virus (ebolavirus Zaire, Mayinga) minigenome

Use

Tags

3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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FLX1.8NFATGFPpd2HS4

Plasmid

#169096

Purpose

Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

Depositor

Geoffrey Owens

Inserts

NFAT enhancer (from IL-4 promoter)
Destabilized green fluorescent protein

Use

Lentiviral

Tags

Expression

Mutation

Promoter

NFAT enhancer + synthetic TATA box

Availability

Academic Institutions and Nonprofits only

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SIN40C.EFS.eGFP.pre

Plasmid

#169276

Purpose

Constitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.GFP.miR30n

Plasmid

#169278

Purpose

Constitutive overexpression of miRNA or shRNA with GFP as reporter fluorescent protein

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.MCS.IRES.GFP

Plasmid

#169280

Purpose

Constitutive overexpression of cDNA with GFP as a reporter fluorescent protein

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.dTomato.miR30n.PGK.sfGFP.P2A.Tet3G

Plasmid

#169282

Purpose

Doxycycline-inducible overexpression of miRNA or shRNA with dTomato as a reporter fluorescent protein

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169283

Purpose

Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.ARID3a.IRES.GFP

Plasmid

#169300

Purpose

Constitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

ARID3A (ARID3A Human)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mArid3a.IRES.GFP

Plasmid

#169309

Purpose

Constitutive overexpression of murine Arid3a cDNA with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.ARID3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169314

Purpose

Doxycycline-inducible expression of human ARID3A cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

ARID3A (ARID3A Human)

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169317

Purpose

Doxycycline-inducible expression of Luciferase cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169318

Purpose

Doxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pTVGFP

Plasmid

#169445

Purpose

A vector with two different MCSs flanking an attP integration site and a floxable pax-GFP marker

Depositor

Jean-Paul Vincent

Article

Yu et al Dev Cell. 2020 Sep 14;54(5):583-592.e5.

Type

Empty backbone

Use

Targeting vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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