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pcDNA3-Casp2-Flag
Plasmid#11811DepositorInsertCaspase-2 (CASP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-CMV-K2M
Plasmid#21164DepositorArticleUseLentiviral; ReprogrammingTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS314-T7-GFP
Plasmid#33130DepositorInsertGFP with TDH3 5' and 3' UTR (gfp )
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPL5606_TEF1*-Cre_ADE2
Plasmid#60932PurposeConstitutive expression vector for Cre-recombinase containing ADE2 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0286
Plasmid#149333PurposeBacterial expression of SENP_EuB proteaseDepositorInsertSENP_EuB protease
UseTagsHis14 and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
HTR1A-Tango
Plasmid#66404PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHTR1A (HTR1A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEH31 Lamp2b HA
Plasmid#168208PurposeMammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p281 TIR1_HA_flox STOP_mH11
Plasmid#164856PurposeCre-inducible TIR1 expression vector for mouse H11 locusDepositorInsertOsTIR1
UseTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-MCM4/6
Plasmid#116951PurposeExpresses human MCM4 and human MCM6DepositorUseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
Plasmid#121966PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.DepositorInsertscFv-GCN4
UseLentiviralTagssuperfold GFP-GB1-NLS-iLIDExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK14_IDG-K
Plasmid#158172PurposeGateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK14 (CDK14 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SP-Cas9 reporter
Plasmid#62733PurposeMammalian CRISPR-SP-Cas9 reporterDepositorInsertCRISPR-SP-Cas9 target seq + tdTomato (out of frame)
UseCRISPR and LentiviralTagsCRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…ExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14
Plasmid#231365PurposeminBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Ple155-CI-EGFP-W3SL_2x-U6-sasgAi14
Plasmid#231366PurposePle155-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minBG-CI-SaCas9-W3SynpA
Plasmid#231369PurposeminBG-driven SaCas9, with short synthetic terminator sequence.DepositorInsertSaCas9
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minCMVe-SCP1-SaCas9-W3SynpA
Plasmid#231370PurposeSCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.DepositorInsertSaCas9
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-ecfp
Plasmid#78463PurposeBroad range bacterial fluorescence labelingDepositorInserteCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-eGFP-W3SL
Plasmid#218742PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)
Plasmid#224489PurposeGateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)DepositorInsertSun1-2xsfGFP-6xMYC-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRZ-CAS9-mCherry
Plasmid#80974PurposeCas9, mCherry expressionDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFW1000
Plasmid#224210PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human Epigenetic Knockout Library
Pooled Library#162256PurposeLentiviral CRISPR library targets human epigenetic genesDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVP073
Plasmid#222076PurposeeforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP074
Plasmid#222077PurposeeforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP075
Plasmid#222078PurposeAeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF1456
Plasmid#143811PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTFEC (TFEC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
HO-Poly-KanMX4-HO
Plasmid#51662Purposeyeast plasmid for integration of target sequence at HO locus, Kan selectionDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
UseTagsExpressionBacterialMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257APromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
XE141 sFRP-1-CS2+
Plasmid#16693DepositorInsertsFRP-1 (Sfrp1 Mouse)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBT378_pattB-pCA-GFP-pA-attB
Plasmid#52554Purposefor PhiC31 integrase-mediated cassette exchangeDepositorInsertsPhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV6-Bmal-luc
Plasmid#68833PurposeExpression of Bmal1-luc in mammalian cellsDepositorInsertBmal1 regulatory sequences (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Vimentin-7
Plasmid#56439PurposeLocalization: Intermediate Filaments, Excitation: 488, Emission: 507DepositorInsertVimentin (VIM Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Casp7-Flag
Plasmid#11815DepositorInsertCaspase 7 (CASP7 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF1-V1-FLAG-IRES-GFP
Plasmid#107387PurposeLentiviral expression of IKZF1-V1-FLAGDepositorInsertIKZF1-V1 (IKZF1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_4D5-High-CAR_RHL002
Plasmid#164825PurposeLentiviral vector for constitutive expression of AntiHER2 4D5-High CARDepositorInsertAntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
psfaMs-Tn5
Plasmid#183637PurposeExpresses anti-mouse nanobody fused with Tn5DepositorInsertanti mouse secondary nanobody fused to Tn5
UseTagsanti-mouse nanobodyExpressionBacterialMutationPromoterAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBOB-VE-cadherin-tdTomato
Plasmid#118733PurposeTo express VE-cadherin tagged with tdTomato on C-terminusDepositorInsertCadherin 5 (CD144) (CDH5 Human)
UseLentiviralTagstdTomatoExpressionMutationI503T (Common SNP-see comments)PromoterCMVAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1aGFP2AKAT7-W
Plasmid#159292PurposeLentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoterDepositorInsertKAT7 (KAT7 Human)
UseLentiviralTagsExpressionMammalianMutationCRISPR sites mutated (gRNA ID. 5 and A10)Promoterhuman EF1a promoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-MCU-GFP
Plasmid#31732DepositorInsertMCU (MCU Human)
UseTagsGFPExpressionMammalianMutation*PromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPA8
Plasmid#19541DepositorInsertheat shock 70kDa protein 8 (HSPA8 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-FBXL11
Plasmid#126543PurposeExpresses GFP-tagged FBXL11 in mammalian cellsDepositorInsertFBXL11 (KDM2A Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterLTRAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGCS-C5(GST)
Plasmid#85728PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTagsGSTExpressionMutationPromoterAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-BZLF1
Plasmid#72637PurposeMammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulatorDepositorInsertBZLF1
UseTagsExpressionMammalianMutationQ163RPromoterAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pM355
Plasmid#173175Purpose(Empty Backbone) Expresses gRNA-12xMBS from hU6 promoterDepositorInsertgRNA-12xMBS backbone
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32-MCM2/7
Plasmid#116949PurposeExpresses human MCM2 and human MCM7DepositorUseTagsThioredoxin, His6ExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-teD-Cit
Plasmid#116041PurposeReporter plasmid for measuring protease activityDepositorInsertteD-Cit
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-REL
Plasmid#27253DepositorInsertREL (REL Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only
