-
Plasmid#131497PurposeEndogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only
-
ABE8.20-d
Plasmid#136301Purposeexpresses ABE8.20-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PPP6C_pLX307
Plasmid#98360PurposeLentiviral expression of PPP6CDepositorInsertPPP6C (PPP6C Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human FLAG-WDR20
Plasmid#217618PurposeMammalian expression of FLAG tagged WDR20DepositorInsertWDR20 (WDR20 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNX25-PX (506-628)
Plasmid#119104PurposeBacterial expression of human phox homology (PX) domain, SNX25-PX (506-628)DepositorInsertSNX25-PX (506-628) (SNX25 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX14-PX (561-686)
Plasmid#119095PurposeBacterial expression of human phox homology (PX) domain, SNX14-PX (561-686)DepositorInsertSNX14-PX (561-686) (SNX14 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSTING (V155M)
Plasmid#214161PurposeTransient expression of gain-of-function STING mutantDepositorInsertSTING (V155M) (STING1 Human)
UseTagsExpressionMammalianMutationV155MPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
ACE2(-1119)-luc
Plasmid#31110PurposeLuciferase reporter construct for human ACE2 promoterDepositorInsertACE2 promoter (ACE2 Human)
UseLuciferaseTagsExpressionMammalianMutationpromoter region -1119 to +103PromoterAvailable sinceOct. 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
p6600 MSCV-IP N-HAonly JUN
Plasmid#34898DepositorInsertJUN (JUN Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ATF6-(S1P-)
Plasmid#32956PurposeFor visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.DepositorInsertATF6 (ATF6 Human)
UseTagsEGFPExpressionMammalianMutationS1Protease site mutation: R415A/R416APromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_SDHB
Plasmid#177981Purposelentiviral vector expressing Cas9 and a sgRNA targeting SDHBDepositorInsertsgRNA targeting SDHB (SDHB Human)
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mClover3-Puromycin
Plasmid#167205PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsmClover3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBE2LB_pLX307
Plasmid#98380PurposeLentiviral expression of UBE2L6DepositorInsertUBE2L6 (UBE2L6 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
AID-BE4max-P2A-GFP
Plasmid#157948PurposeMammalian expression of AID-BE4max-P2A-GFPDepositorInsertAID-BE4max
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp12
Plasmid#157709Purposemammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoterDepositorInsertNsp12 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp8
Plasmid#157694Purposemammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertNsp8 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLeo-A-a2-GEMS(JAK-STAT)
Plasmid#209110PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)DepositorInsertA-a2-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l4-G'-IRES-EmGFP
Plasmid#209127PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFPDepositorInsertSUMO-A'-l4-G'-IRES-EmGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l4-G-IRES-EmGFP
Plasmid#209126PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFPDepositorInsertSUMO-A'-l4-G-IRES-EmGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp8-HF
Plasmid#157696Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertNsp8-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits