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Plasmid#51590Purposeexpresses myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 69 to Alanine; also RNAi resistantPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGLx2-set1-H1017K
Plasmid#53258PurposeGAL driven LexA-tagged SET1-catalytically dead (H1017K)DepositorInsertSET1
UseTagsLexAExpressionYeastMutationH1017K mutation - catalytically deadPromoterGAL1/10Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 342
Plasmid#53012PurposePLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfpDepositorInsertsmCherry
araC
ompC
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains -81 to +36 relative to start codonPromoterPlar, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2 short
Plasmid#51596Purposeexpresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)DepositorInsertRem2 (Rem2 Rat)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S308A
Plasmid#51592Purposeexpresses myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 308 to Alanine; also RNAi resistantPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S241A
Plasmid#51591Purposeexpresses myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 241 to Alanine; also RNAi resistantPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 R236A/L263A
Plasmid#51589Purposeexpresses RNAiR myc-tagged Rem2 non-Ca channel interactingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationR236A and L263A; silent mutations at shRNA 1 and …PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S69A/S334A
Plasmid#51588Purposeexpresses RNAiR myc-tagged Rem2 non-14-3-3 interactingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationS69A and S334A; silent mutations at shRNA 1 and 2…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-fly Arp11
Plasmid#51399Purposeexpression of full-length Drosophila Arp11 in mammalian cellsDepositorInsertArp11 (Arp10 Fly)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
MR015-PHOX2BLeftTALEN_NN
Plasmid#49450PurposeLeft TALEN for PHOX2B with NNDepositorInsertTALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
MR015-PHOX2BRightTALEN_NN
Plasmid#49449PurposeRight TALEN for PHOX2B with NNDepositorInsertTALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
MR015-PHOX2BRightTALEN_NK
Plasmid#49478PurposeRight TALEN for PHOX2B with NKDepositorInsertTALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
MR015-PHOX2BLeftTALEN_NK
Plasmid#49477PurposeLeft TALEN for PHOX2B with NKDepositorInsertTALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSS63
Plasmid#47951PurposertTA positive feedback loop with single tet operator in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3531
Plasmid#47250DepositorInsertZebrafishCommunity-retinitispigmentosaGTPaseregulatorbORF15-right
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3530
Plasmid#47249DepositorInsertZebrafishCommunity-retinitispigmentosaGTPaseregulatorbORF15-left
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFS320-USP44-catDead
Plasmid#43901DepositorInsertUSP44 (USP44 Human)
UseTagsMYCExpressionMammalianMutationC282A, T91APromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only