Showing: 11681 - 11700 of 14149 results
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Plasmid#167354PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cellsDepositorInsertE3 ubiquitin-protein ligase RNF216
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e VPR
Plasmid#188516PurposeExpresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)DepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-VPRExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR TRF2
Plasmid#185443PurposeExpresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertTRF2 (TERF2 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR POT1
Plasmid#185444PurposeExpresses human POT1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertPOT1 (POT1 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR TRF1
Plasmid#185445PurposeExpresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertTRF1 (TERF1 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-mKate2-IRES-Hph
Plasmid#183616PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.DepositorInsertmKate2
UseTagsHuman EMD transmembrane domain and NLSExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-HistoneH2B-L2
Plasmid#186357PurposeEntry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertHistone H2B (H2BC11 Human)
UseExpression of a tagged nuclear markerTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCas4_1_2_VA
Plasmid#186438PurposeEncodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCas4_1_Δ2_VA
Plasmid#186439PurposeEncodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCasΔ4_1_2_VA
Plasmid#186440PurposeEncodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCas-mut4_1_2_VA
Plasmid#186441PurposeEncodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1_2_VB
Plasmid#186442PurposeEncodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1Δ2_VB
Plasmid#186443PurposeEncodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1
UseCRISPRTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_ATG4B_BamHI_NotI
Plasmid#190862PurposePlasmid for the expression and purification of of ATG4B. Internal reference: SMC1392DepositorInsertATG4B (ATG4B Human)
UseTagsGST, Thrombin Cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEY26
Plasmid#191031Purposecho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertcho-1(3.3-2.6k) (cho-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailabilityAcademic Institutions and Nonprofits only -
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pEY43
Plasmid#191047Purposeeat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInserteat-4(prom6-1) (eat-4 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailabilityAcademic Institutions and Nonprofits only -
Showing: 11681 - 11700 of 14149 results