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Plasmid#204253PurposeBacterial expression of G121F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M91Q wgMDH
Plasmid#204250PurposeBacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationM91QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D87L wgMDH
Plasmid#204248PurposeBacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationD87LPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126G wgMDH
Plasmid#204260PurposeBacterial expression of P126G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP126GPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123G wgMDH
Plasmid#204256PurposeBacterial expression of P123G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123GPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123F wgMDH
Plasmid#204255PurposeBacterial expression of P123F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKS2delARMec
Plasmid#130860PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of armadillo domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2del4exp
Plasmid#130861PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of terminal four amino acidsPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delKASHexp
Plasmid#130862PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of KASH domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delTMexp
Plasmid#130863PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of transmembrane domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delARMexp
Plasmid#130864PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of armadillo domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2del4ec
Plasmid#130857PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of terminal four amino acidsPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delKASHec
Plasmid#130858PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationeGFP-FLAG-HAPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDropIn-T2A-ZpGal4DBD
Plasmid#199199PurposeInsert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-ZpGal4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-p65Zp
Plasmid#199200PurposeInsert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-p65Zp
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYG205
Plasmid#200836PurposeTranscribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivativesDepositorInsertrapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP-BADAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-DmKHC[1-421]-1xGFP
Plasmid#196973PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGFP and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-SNAP-DmKHC[1-421]
Plasmid#196976PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GSTDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGST and SNAPExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-THTAP-CALPAIN-7 1-165
Plasmid#180610PurposeBacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129DepositorInsertCALPAIN-7
UseTagsHIS-GSTExpressionBacterialMutationResidues 1-65PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GRE
Plasmid#172336PurposeGal4 Response elementDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGK/CMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only