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Plasmid#113130PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 for gRNA expression, RSV for GFP expressionAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST1374-H840A-GCN4
Plasmid#113027PurposeExpresses H840A-GCN4 in mammalian cellsDepositorInsertH840A-GCN4 (GCN4 S. pyogenes, Budding Yeast)
UseTagsExpressionMammalianMutationD839A, H840A, N863APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sema6b(L)-AP-His
Plasmid#72039PurposeExpresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6b (Sema6b Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxna3-AP-His
Plasmid#71998PurposeExpresses the extracellular region of the PlexinA3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxna3 (Plxna3 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC73
Plasmid#62341PurposesgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cellsDepositorInsertssgRNA
COM-KRAB
UseLentiviralTagsKRABExpressionMammalianMutationTargets sv40 promoter, sequence: GAATAGCTCAGAGGCC…PromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ntng2.b-AP-His
Plasmid#71995PurposeExpresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNtng2.b (Ntng2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 MUT
Plasmid#110124PurposeExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activityDepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutation4 mutations (KSLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sdk1-Fc-His
Plasmid#72134PurposeExpresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSdk1 (Sdk1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mqg*
Plasmid#21175DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationPoint mutation at nucleotide 6163 (numbering acco…PromoterAvailable sinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mal*
Plasmid#21172DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationPoint mutation at nucleotide 5114 (numbering acco…PromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Farnesyl-5
Plasmid#57613PurposeLocalization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertFarnesyl (HRAS Human)
UseTagstdEosExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Sema4d-AP-His
Plasmid#72031PurposeExpresses the extracellular region of the Sema4D protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema4d (Sema4d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE254 pCDNA3.1(zeo)-hDsh3-497-716
Plasmid#16755DepositorInsertFLAG-497-716-Dsh3 (DVL3 Human)
UseTagsflagExpressionMammalianMutationaa 497-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorInsertfull length me31B (me31B Fly)
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-Actin-C-18
Plasmid#56400PurposeLocalization: Actin, Excitation: 530, Emission: 541DepositorInsertActin (ACTB Human)
UseTagsmPapaya1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.5-Fc-His
Plasmid#72102PurposeExpresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNrp2.5 (Nrp2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23110-mRFP1-331Bb
Plasmid#78277PurposemRFP1 behind constitutive promoter J23110 in pSEVA331Bb backboneDepositorInsertJ23110-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-luciferase
Plasmid#113352Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-CRY2PHR-SID4X
Plasmid#63663PurposeAAV expression of CRYS2PHR-SID4XDepositorInsertSID4X-CYR2PHR
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -