ESR1-Tol2
(Plasmid
#113382)
-
Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 113382 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneTol2 reporter vector
-
Vector typeTol2 reporter
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameESR1-enhancer
-
SpeciesH. sapiens (human)
-
Entrez GeneESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
ESR1-Tol2 was a gift from Ralf Jauch (Addgene plasmid # 113382 ; http://n2t.net/addgene:113382 ; RRID:Addgene_113382) -
For your References section:
DNA-mediated dimerization on a compact sequence signature controls enhancer engagement and regulation by FOXA1. Wang X, Srivastava Y, Jankowski A, Malik V, Wei Y, Del Rosario RC, Cojocaru V, Prabhakar S, Jauch R. Nucleic Acids Res. 2018 Jun 20;46(11):5470-5486. doi: 10.1093/nar/gky259. 10.1093/nar/gky259 PubMed 29669022