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Plasmid#209330PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mTagBFP2 fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pLCS108
Plasmid#209329PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCS99
Plasmid#209327PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of untagged protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk19/kcnk12l
Plasmid#209001PurposeRiboprobe generationDepositorInsertkcnk19 (kcnk12l Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk2b
Plasmid#208984PurposeRiboprobe generationDepositorInsertkcnk2b (kcnk2b Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk4a
Plasmid#208987PurposeRiboprobe generationDepositorInsertkcnk4a (kcnk4a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-B
Plasmid#196987PurposeFor SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.DepositorInsertSpy gRNA cassette
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpltetO1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0520 (pDEST, TRP1)
Plasmid#210481PurposepDEST vector with TRP1 marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseDestination vector for gateway cloningTagsExpressionMutationPromoterADH1Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0604 (pDEST, HygR)
Plasmid#210483PurposepDEST vector with HygR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0603 (pDEST, NatR)
Plasmid#210482PurposepDEST vector with NatR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCD-Ugi22
Plasmid#207004PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)DepositorInsertUtricularia gibba insulator 22
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCD-Ugi21
Plasmid#207005PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)DepositorInsertUtricularia gibba insulator 21
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF CAG mc2 IRES neo
Plasmid#206232PurposeFor expressing cloned exons as circular RNA.DepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0R_lacZ
Plasmid#202095PurposeReceiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJS506
Plasmid#200712PurposePromoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeastDepositorInsertPMA1 promoter (PMA1 Budding Yeast)
UseSynthetic Biology; Modular cloningTagsExpressionMutationdomesticated for MoClo cloning (removal of intern…PromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg24 1B
Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
UseTags6His-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg32 [200-341] 1B
Plasmid#190431PurposeRecombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.DepositorInsertAtg32
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-ADCK5-V5-miniTurbo_IDG-K
Plasmid#189909PurposeGateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertADCK5 (ADCK5 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL70
Plasmid#175897PurposepENTRY for Gateway LR reactionDepositorInsertID#13
UsePentry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL201
Plasmid#175919PurposepENTRY for Gateway LR reactionDepositorInsertID#88
UsePentry cloneTagsExpressionMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only